caa a22 4500 605504393 CHVBK 20210128100615.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6291-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6291-5 The two putative comS homologs of the biotechnologically important Bacillus licheniformis do not contribute to competence development [Elektronische Daten] [Mareike Jakobs, Kerstin Hoffmann, Heiko Liesegang, Sonja Volland, Friedhelm Meinhardt] In Bacillus subtilis, natural genetic competence is subject to complex genetic regulation and quorum sensing dependent. Upon extracellular accumulation of the peptide-pheromone ComX, the membrane-bound sensor histidine kinase ComP initiates diverse signaling pathways by activating—among others—DegQ and ComS. While DegQ favors the expression of extracellular enzymes rather than competence development, ComS is crucial for competence development as it prevents proteolytic degradation of ComK, the key transcriptional activator of all genes required for the uptake and integration of DNA. In Bacillus licheniformis, ComX/ComP sensed cell density negatively influences competence development, suggesting differences from the quorum-sensing-dependent control mechanism in Bacillus subtilis. Here, we show that each of six investigated strains possesses both of two different, recently identified putative comS genes. When expressed from an inducible promoter, none of the comS candidate genes displayed an impact on competence development neither in B. subtilis nor in B. licheniformis. Moreover, disruption of the genes did not reduce transformation efficiency. While the putative comS homologs do not contribute to competence development, we provide evidence that the degQ gene as for B. subtilis negatively influences genetic competency in B. licheniformis. Springer-Verlag Berlin Heidelberg, 2014 Natural genetic competence nationallicence Bacillus licheniformis nationallicence Quorum sensing nationallicence ComS nationallicence DegQ nationallicence Jakobs Mareike Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, 48149, Münster, Germany aut Hoffmann Kerstin Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, 48149, Münster, Germany aut Liesegang Heiko Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Georg-August-University Göttingen, Grisebachstrasse 8, 37077, Göttingen, Germany aut Volland Sonja Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Georg-August-University Göttingen, Grisebachstrasse 8, 37077, Göttingen, Germany aut Meinhardt Friedhelm Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, 48149, Münster, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2255-2266 0175-7598 99:5<2255 2015 99 253 https://doi.org/10.1007/s00253-014-6291-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6291-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jakobs Mareike Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, 48149, Münster, Germany aut NATIONALLICENCE 700 1- Hoffmann Kerstin Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, 48149, Münster, Germany aut NATIONALLICENCE 700 1- Liesegang Heiko Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Georg-August-University Göttingen, Grisebachstrasse 8, 37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Volland Sonja Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Georg-August-University Göttingen, Grisebachstrasse 8, 37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Meinhardt Friedhelm Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, 48149, Münster, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2255-2266 0175-7598 99:5<2255 2015 99 253