caa a22 4500 605504423 CHVBK 20210128100616.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6044-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6044-5 Analysis of the biological activities of Saccharomyces cerevisiae expressing intracellular EGF, extracellular EGF, and tagged EGF in early-weaned rats [Elektronische Daten] [Shujin Wang, Lin Zhou, Huina Chen, Yangchun Cao, Zhengfan Zhang, Jiabao Yang, Yanlin Huang, Chunhua Guo] A growing number of studies suggest that epidermal growth factor (EGF) plays an important role in early-weaned animals. The objective of this experiment was to compare the biological activity of intracellularly expressed EGF (IE-EGF), extracellularly expressed EGF (EE-EGF), and tagged EGF (T-EGF) from Saccharomyces cerevisiae (S. cerevisiae) both in vivo and in vitro. Strains of S. cerevisiae expressing IE-EGF, EE-EGF, and T-EGF were designated INVSc1-IE(+), INVSc1-EE(+), and INVSc1-TE(−), respectively. The production performance, intestinal development, physio-biochemical indexes, and immunological function of early-weaned rats were measured in vivo to evaluate the biological activity of IE-EGF, EE-EGF, and T-EGF. In addition, the proliferation of rat enterocyte was also measured in vitro. In the in vivo experiment, the recombinant S. cerevisiae was shown to survive throughout the intestinal tract. The production performance (e.g., body weight) and intestinal development (e.g., mean villous height, crypt depth, total protein, DNA, and RNA) of the rats were significantly enhanced in the INVSc1-IE(+) group compared with the INVSc1-EE(+) and INVSc1-TE(−) groups (P < 0.05). However, the levels of lactate dehydrogenase (LDH), immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) showed no difference in the INVSc1-IE(+) group compared to the INVSc1-EE(+) and INVSc1-TE(−) groups (P > 0.05), with the only significant difference being found for creatine kinase (CK) (P < 0.05). In the in vitro experiment, the proliferation of enterocyte was significantly stimulated by both IE-EGF and EE-EGF compared with T-EGF (P < 0.05). Herein, IE-EGF is more suitable for application to early-weaned animals compared with EE-EGF and T-EGF. Springer-Verlag Berlin Heidelberg, 2014 Biological activity nationallicence Early-weaned rat nationallicence Epidermal growth factor nationallicence Protein expressing nationallicence Saccharomyces cerevisiae nationallicence Wang Shujin College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu City, People's Republic of China aut Zhou Lin Shenzhen Premixinve Nutrition Co., LTD, 518103, Shenzhen, People's Republic of China aut Chen Huina College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu City, People's Republic of China aut Cao Yangchun Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut Zhang Zhengfan College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu City, People's Republic of China aut Yang Jiabao Animal Science Research Institute of Sichuan Province, 610041, Chengdu, People's Republic of China aut Huang Yanlin College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu City, People's Republic of China aut Guo Chunhua College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu City, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2179-2189 0175-7598 99:5<2179 2015 99 253 https://doi.org/10.1007/s00253-014-6044-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6044-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Shujin College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu City, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Lin Shenzhen Premixinve Nutrition Co., LTD, 518103, Shenzhen, People's Republic of China aut NATIONALLICENCE 700 1- Chen Huina College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu City, People's Republic of China aut NATIONALLICENCE 700 1- Cao Yangchun Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Zhengfan College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu City, People's Republic of China aut NATIONALLICENCE 700 1- Yang Jiabao Animal Science Research Institute of Sichuan Province, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Huang Yanlin College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu City, People's Republic of China aut NATIONALLICENCE 700 1- Guo Chunhua College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu City, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2179-2189 0175-7598 99:5<2179 2015 99 253