caa a22 4500 605504466 CHVBK 20210128100616.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-015-6388-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6388-5 Genomic analysis of Brevibacillus thermoruber 423 reveals its biotechnological and industrial potential [Elektronische Daten] [Songul Yildiz, Nadja Radchenkova, Kazim Arga, Margarita Kambourova, Ebru Toksoy Oner] Brevibacillus thermoruber 423 is a Gram-positive, motile, red-pigmented, spore-forming, aerobic, and thermophilic bacterium that is known to produce high levels of exopolysaccharide (EPS) with many potential uses in food, feed, cosmetics, and pharmaceutical and chemical industries. This bacterium not only is among the limited number of reported thermophilic EPS producers but also exceeds other thermophilic producers in light of the high level of polymer synthesis. By a systems-based approach, whole-genome analysis of this bacterium was performed to gain more insight about the biological mechanisms and whole-genome organization of thermophilic EPS producers and hence to develop rational strategies for the genetic and metabolic optimization of EPS production. Also with this study, the first genome analysis was performed on a thermophilic Brevibacillus species. Essential genes associated with EPS biosynthesis were detected by genome annotation, and together with experimental evidences, a hypothetical mechanism for EPS biosynthesis was generated. B. thermoruber 423 was found to have many potential applications in biotechnology and industry because of its capacity to utilize xylose and to produce EPS, isoprenoids, ethanol/butanol, lipases, proteases, cellulase, and glucoamylase enzymes as well as its resistance to arsenic. Springer-Verlag Berlin Heidelberg, 2015 Brevibacillus thermoruber nationallicence Thermophiles nationallicence Genome nationallicence Next-generation sequencing nationallicence Exopolysaccharide nationallicence Yildiz Songul IBSB, Department of Bioengineering, Marmara University, 34722, Istanbul, Turkey aut Radchenkova Nadja Department of Extremophilic Bacteria, Institute of Microbiology, BAS, Sofia, Bulgaria aut Arga Kazim IBSB, Department of Bioengineering, Marmara University, 34722, Istanbul, Turkey aut Kambourova Margarita Department of Extremophilic Bacteria, Institute of Microbiology, BAS, Sofia, Bulgaria aut Toksoy Oner Ebru IBSB, Department of Bioengineering, Marmara University, 34722, Istanbul, Turkey aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2277-2289 0175-7598 99:5<2277 2015 99 253 https://doi.org/10.1007/s00253-015-6388-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6388-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yildiz Songul IBSB, Department of Bioengineering, Marmara University, 34722, Istanbul, Turkey aut NATIONALLICENCE 700 1- Radchenkova Nadja Department of Extremophilic Bacteria, Institute of Microbiology, BAS, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Arga Kazim IBSB, Department of Bioengineering, Marmara University, 34722, Istanbul, Turkey aut NATIONALLICENCE 700 1- Kambourova Margarita Department of Extremophilic Bacteria, Institute of Microbiology, BAS, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Toksoy Oner Ebru IBSB, Department of Bioengineering, Marmara University, 34722, Istanbul, Turkey aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2277-2289 0175-7598 99:5<2277 2015 99 253