Batch, design optimization, and DNA sequencing study for continuous 1,3-propanediol production from waste glycerol by a soil-based inoculum
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6259-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6259-5 | ||
| 245 | 0 | 0 | |a Batch, design optimization, and DNA sequencing study for continuous 1,3-propanediol production from waste glycerol by a soil-based inoculum |h [Elektronische Daten] |c [Baishali Kanjilal, Iman Noshadi, Eddy Bautista, Ranjan Srivastava, Richard Parnas] |
| 520 | 3 | |a 1,3-propanediol (1,3-PD) was produced with a robust fermentation process using waste glycerol feedstock from biodiesel production and a soil-based bacterial inoculum. An iterative inoculation method was developed to achieve independence from soil and selectively breed bacterial populations capable of glycerol metabolism to 1,3-PD. The inoculum showed high resistance to impurities in the feedstock. 1,3-PD selectivity and yield in batch fermentations was optimized by appropriate nutrient compositions and pH control. The batch yield of 1,3-PD was maximized to ~0.7mol/mol for industrial glycerol which was higher than that for pure glycerin. 16S rDNA sequencing results show a systematic selective enrichment of 1,3-PD producing bacteria with iterative inoculation and subsequent process control. A statistical design of experiments was carried out on industrial glycerol batches to optimize conditions, which were used to run two continuous flow stirred-tank reactor (CSTR) experiments over a period of >500h each. A detailed analysis of steady states at three dilution rates is presented. Enhanced specific 1,3-PD productivity was observed with faster dilution rates due to lower levels of solvent degeneration. 1,3-PD productivity, specific productivity, and yield of 1.1g/lhr, 1.5g/ghr, and 0.6mol/mol of glycerol were obtained at a dilution rate of 0.1h−1which is bettered only by pure strains in pure glycerin feeds. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a 1,3-propanediol |2 nationallicence | |
| 690 | 7 | |a Mixed bacterial inoculum |2 nationallicence | |
| 690 | 7 | |a CSTR |2 nationallicence | |
| 690 | 7 | |a Batch fermentation |2 nationallicence | |
| 690 | 7 | |a Design of experiment |2 nationallicence | |
| 690 | 7 | |a 16S sequencing |2 nationallicence | |
| 700 | 1 | |a Kanjilal |D Baishali |u Institute of Materials Science, University of Connecticut, 97 N. Eagleville Rd., 06269, Storrs, CT, USA |4 aut | |
| 700 | 1 | |a Noshadi |D Iman |u Department of Chemical and Biomolecular Engineering, University of Connecticut, 191 Auditorium Rd., 06269, Storrs, CT, USA |4 aut | |
| 700 | 1 | |a Bautista |D Eddy |u Department of Chemical and Biomolecular Engineering, University of Connecticut, 191 Auditorium Rd., 06269, Storrs, CT, USA |4 aut | |
| 700 | 1 | |a Srivastava |D Ranjan |u Department of Chemical and Biomolecular Engineering, University of Connecticut, 191 Auditorium Rd., 06269, Storrs, CT, USA |4 aut | |
| 700 | 1 | |a Parnas |D Richard |u Institute of Materials Science, University of Connecticut, 97 N. Eagleville Rd., 06269, Storrs, CT, USA |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/5(2015-03-01), 2105-2117 |x 0175-7598 |q 99:5<2105 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6259-5 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6259-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kanjilal |D Baishali |u Institute of Materials Science, University of Connecticut, 97 N. Eagleville Rd., 06269, Storrs, CT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Noshadi |D Iman |u Department of Chemical and Biomolecular Engineering, University of Connecticut, 191 Auditorium Rd., 06269, Storrs, CT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bautista |D Eddy |u Department of Chemical and Biomolecular Engineering, University of Connecticut, 191 Auditorium Rd., 06269, Storrs, CT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Srivastava |D Ranjan |u Department of Chemical and Biomolecular Engineering, University of Connecticut, 191 Auditorium Rd., 06269, Storrs, CT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Parnas |D Richard |u Institute of Materials Science, University of Connecticut, 97 N. Eagleville Rd., 06269, Storrs, CT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/5(2015-03-01), 2105-2117 |x 0175-7598 |q 99:5<2105 |1 2015 |2 99 |o 253 | ||