caa a22 4500 605504474 CHVBK 20210128100616.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6259-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6259-5 Batch, design optimization, and DNA sequencing study for continuous 1,3-propanediol production from waste glycerol by a soil-based inoculum [Elektronische Daten] [Baishali Kanjilal, Iman Noshadi, Eddy Bautista, Ranjan Srivastava, Richard Parnas] 1,3-propanediol (1,3-PD) was produced with a robust fermentation process using waste glycerol feedstock from biodiesel production and a soil-based bacterial inoculum. An iterative inoculation method was developed to achieve independence from soil and selectively breed bacterial populations capable of glycerol metabolism to 1,3-PD. The inoculum showed high resistance to impurities in the feedstock. 1,3-PD selectivity and yield in batch fermentations was optimized by appropriate nutrient compositions and pH control. The batch yield of 1,3-PD was maximized to ~0.7mol/mol for industrial glycerol which was higher than that for pure glycerin. 16S rDNA sequencing results show a systematic selective enrichment of 1,3-PD producing bacteria with iterative inoculation and subsequent process control. A statistical design of experiments was carried out on industrial glycerol batches to optimize conditions, which were used to run two continuous flow stirred-tank reactor (CSTR) experiments over a period of >500h each. A detailed analysis of steady states at three dilution rates is presented. Enhanced specific 1,3-PD productivity was observed with faster dilution rates due to lower levels of solvent degeneration. 1,3-PD productivity, specific productivity, and yield of 1.1g/lhr, 1.5g/ghr, and 0.6mol/mol of glycerol were obtained at a dilution rate of 0.1h−1which is bettered only by pure strains in pure glycerin feeds. Springer-Verlag Berlin Heidelberg, 2014 1,3-propanediol nationallicence Mixed bacterial inoculum nationallicence CSTR nationallicence Batch fermentation nationallicence Design of experiment nationallicence 16S sequencing nationallicence Kanjilal Baishali Institute of Materials Science, University of Connecticut, 97 N. Eagleville Rd., 06269, Storrs, CT, USA aut Noshadi Iman Department of Chemical and Biomolecular Engineering, University of Connecticut, 191 Auditorium Rd., 06269, Storrs, CT, USA aut Bautista Eddy Department of Chemical and Biomolecular Engineering, University of Connecticut, 191 Auditorium Rd., 06269, Storrs, CT, USA aut Srivastava Ranjan Department of Chemical and Biomolecular Engineering, University of Connecticut, 191 Auditorium Rd., 06269, Storrs, CT, USA aut Parnas Richard Institute of Materials Science, University of Connecticut, 97 N. Eagleville Rd., 06269, Storrs, CT, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2105-2117 0175-7598 99:5<2105 2015 99 253 https://doi.org/10.1007/s00253-014-6259-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6259-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kanjilal Baishali Institute of Materials Science, University of Connecticut, 97 N. Eagleville Rd., 06269, Storrs, CT, USA aut NATIONALLICENCE 700 1- Noshadi Iman Department of Chemical and Biomolecular Engineering, University of Connecticut, 191 Auditorium Rd., 06269, Storrs, CT, USA aut NATIONALLICENCE 700 1- Bautista Eddy Department of Chemical and Biomolecular Engineering, University of Connecticut, 191 Auditorium Rd., 06269, Storrs, CT, USA aut NATIONALLICENCE 700 1- Srivastava Ranjan Department of Chemical and Biomolecular Engineering, University of Connecticut, 191 Auditorium Rd., 06269, Storrs, CT, USA aut NATIONALLICENCE 700 1- Parnas Richard Institute of Materials Science, University of Connecticut, 97 N. Eagleville Rd., 06269, Storrs, CT, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2105-2117 0175-7598 99:5<2105 2015 99 253