caa a22 4500 605504482 CHVBK 20210128100616.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6125-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6125-5 Mixotrophic metabolism of Chlorella sorokiniana and algal-bacterial consortia under extended dark-light periods and nutrient starvation [Elektronische Daten] [Cynthia Alcántara, Carolina Fernández, Pedro García-Encina, Raúl Muñoz] Microalgae harbor a not fully exploited industrial and environmental potential due to their high metabolic plasticity. In this context, a better understanding of the metabolism of microalgae and microalgal-bacterial consortia under stress conditions is essential to optimize any waste-to-value approach for their mass cultivation. This work constitutes a fundamental study of the mixotrophic metabolism under stress conditions of an axenic culture of Chlorella sorokiniana and a microalgal-bacterial consortium using carbon, nitrogen, and phosphorous mass balances. The hydrolysis of glucose into volatile fatty acids (VFA) during dark periods occurred only in microalgal-bacterial cultures and resulted in organic carbon removals in the subsequent illuminated periods higher than in C. sorokiniana cultures, which highlighted the symbiotic role of bacterial metabolism. Acetic acid was preferentially assimilated over glucose and inorganic carbon by C. sorokiniana and by the microalgal-bacterial consortium during light periods. N-NH4 + and P-PO4 −3 removals in the light stages decreased at decreasing duration of the dark stages, which suggested that N and P assimilation in microalgal-bacterial cultures was proportional to the carbon available as VFA to produce new biomass. Unlike microalgal-bacterial cultures, C. sorokiniana released P-PO4 −3 under anaerobic conditions, but this excretion was not related to polyhydroxybutyrate accumulation. Finally, while no changes were observed in the carbohydrate, lipid and protein content during repeated extended dark-light periods, nutrient deprivation boosted both C-acetate and C-glucose assimilation and resulted in significantly high biomass productivities and carbohydrate contents in both C. sorokiniana and the microalgal-bacterial cultures. Springer-Verlag Berlin Heidelberg, 2014 Algal-bacterial consortium nationallicence Bioremediation nationallicence C. sorokiniana nationallicence Extended dark-light periods nationallicence Mass balances nationallicence Nutrient deprivation nationallicence Alcántara Cynthia Department of Chemical Engineering and Environmental Technology, Valladolid University, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut Fernández Carolina Department of Chemical Engineering and Environmental Technology, Valladolid University, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut García-Encina Pedro Department of Chemical Engineering and Environmental Technology, Valladolid University, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut Muñoz Raúl Department of Chemical Engineering and Environmental Technology, Valladolid University, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2393-2404 0175-7598 99:5<2393 2015 99 253 https://doi.org/10.1007/s00253-014-6125-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6125-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alcántara Cynthia Department of Chemical Engineering and Environmental Technology, Valladolid University, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut NATIONALLICENCE 700 1- Fernández Carolina Department of Chemical Engineering and Environmental Technology, Valladolid University, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut NATIONALLICENCE 700 1- García-Encina Pedro Department of Chemical Engineering and Environmental Technology, Valladolid University, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut NATIONALLICENCE 700 1- Muñoz Raúl Department of Chemical Engineering and Environmental Technology, Valladolid University, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2393-2404 0175-7598 99:5<2393 2015 99 253