caa a22 4500 605504520 CHVBK 20210128100616.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6141-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6141-5 Aerobic and nitrite-dependent methane-oxidizing microorganisms in sediments of freshwater lakes on the Yunnan Plateau [Elektronische Daten] [Yong Liu, Jingxu Zhang, Lei Zhao, Yuzhao Li, Yuyin Yang, Shuguang Xie] Both aerobic methane-oxidizing bacteria (MOB) and nitrite-dependent anaerobic methane oxidation (n-damo) bacteria can play an important role in mitigating the methane emission produced in anoxic sediment layers to the atmosphere. However, the environmental factors regulating the distribution of these methane-oxidizing microorganisms in lacustrine ecosystems remain essentially unclear. The present study investigated the distribution of aerobic MOB and n-damo bacteria in sediments of various freshwater lakes on the Yunnan Plateau (China). Quantitative PCR assay and clone library analysis illustrated the spatial variations in the abundances and structures of aerobic MOB and n-damo bacterial communities. Type I MOB (Methylosoma and Methylobacter) and type II MOB (Methylocystis) were detected, while type I MOB was more abundant than type II MOB. Lake sediments n-damo bacterial communities were composed of novel Methylomirabilis oxyfera-like pmoA genes. Lake sediments in the same geographic region could share a relatively similar aerobic MOB community structure. Moreover, Pearson's correlation analysis indicated that n-damo pmoA gene diversity showed a positive correlation with the ratio of organic matter to total nitrogen in lake sediment. Springer-Verlag Berlin Heidelberg, 2014 Methane-oxidizing bacteria nationallicence Nitrite-dependent anaerobic methane oxidation nationallicence High latitude nationallicence Lake nationallicence Sediment nationallicence Liu Yong Key Laboratory of Water and Sediment Sciences (Ministry of Education), College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut Zhang Jingxu State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut Zhao Lei Yunnan Key Laboratory of Pollution Process and Management of Plateau Lake-Watershed, 650034, Kunming, China aut Li Yuzhao Key Laboratory of Water and Sediment Sciences (Ministry of Education), College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut Yang Yuyin State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut Xie Shuguang State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2371-2381 0175-7598 99:5<2371 2015 99 253 https://doi.org/10.1007/s00253-014-6141-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6141-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Yong Key Laboratory of Water and Sediment Sciences (Ministry of Education), College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Zhang Jingxu State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Zhao Lei Yunnan Key Laboratory of Pollution Process and Management of Plateau Lake-Watershed, 650034, Kunming, China aut NATIONALLICENCE 700 1- Li Yuzhao Key Laboratory of Water and Sediment Sciences (Ministry of Education), College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Yang Yuyin State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Xie Shuguang State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2371-2381 0175-7598 99:5<2371 2015 99 253