caa a22 4500 605504547 CHVBK 20210128100617.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-015-6397-4 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6397-4 Impacts of CO2 concentration on growth, lipid accumulation, and carbon-concentrating-mechanism-related gene expression in oleaginous Chlorella [Elektronische Daten] [Jianhua Fan, Hui Xu, Yuanchan Luo, Minxi Wan, Jianke Huang, Weiliang Wang, Yuanguang Li] Biodiesel production by microalgae with photosynthetic CO2 biofixation is thought to be a feasible way in the field of bioenergy and carbon emission reduction. Knowledge of the carbon-concentrating mechanism plays an important role in improving microalgae carbon fixation efficiency. However, little information is available regarding the dramatic changes of cells suffered upon different environmental factors, such as CO2 concentration. The aim of this study was to investigate the growth, lipid accumulation, carbon fixation rate, and carbon metabolism gene expression under different CO2 concentrations in oleaginous Chlorella. It was found that Chlorella pyrenoidosa grew well under CO2 concentrations ranging from 1 to 20%. The highest biomass and lipid productivity were 4.3g/L and 107mg/L/day under 5% CO2 condition. Switch from high (5%) to low (0.03%, air) CO2 concentration showed significant inhibitory effect on growth and CO2 fixation rate. The amount of the saturated fatty acids was increased obviously along with the transition. Low CO2 concentration (0.03%) was suitable for the accumulation of saturated fatty acids. Reducing the CO2 concentration could significantly decrease the polyunsaturated degree in fatty acids. Moreover, the carbon-concentrating mechanism-related gene expression revealed that most of them, especially CAH2, LCIB, and HLA3, had remarkable change after 1, 4, and 24h of the transition, which suggests that Chlorella has similar carbon-concentrating mechanism with Chlamydomonas reinhardtii. The findings of the present study revealed that C. pyrenoidosa is an ideal candidate for mitigating CO2 and biodiesel production and is appropriate as a model for mechanism research of carbon sequestration. Springer-Verlag Berlin Heidelberg, 2015 Chlorella nationallicence CO2 nationallicence Growth nationallicence Lipid nationallicence Carbon-concentrating mechanism nationallicence Gene expression nationallicence Fan Jianhua State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut Xu Hui State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut Luo Yuanchan State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut Wan Minxi State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut Huang Jianke State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut Wang Weiliang State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut Li Yuanguang State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2451-2462 0175-7598 99:5<2451 2015 99 253 https://doi.org/10.1007/s00253-015-6397-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6397-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fan Jianhua State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Xu Hui State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Luo Yuanchan State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wan Minxi State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Huang Jianke State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wang Weiliang State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Li Yuanguang State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 301 Mail Box, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2451-2462 0175-7598 99:5<2451 2015 99 253