caa a22 4500 605504563 CHVBK 20210128100617.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6062-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6062-3 Characterization of the starvation-induced chitinase CfcA and α-1,3-glucanase AgnB of Aspergillus niger [Elektronische Daten] [Jolanda van Munster, Justyna Dobruchowska, Ruud Veloo, Lubbert Dijkhuizen, Marc van der Maarel] The common saprophyte Aspergillus niger may experience carbon starvation in nature as well as during industrial fermentations. Starvation survival strategies, such as conidiation or the formation of exploratory hyphae, require energy and building blocks, which may be supplied by autolysis. Glycoside hydrolases are key effectors of autolytic degradation of fungal cell walls, but knowledge on their identity and functionality is still limited. We recently identified agnB and cfcA as two genes encoding carbohydrate-active enzymes that had notably increased transcription during carbon starvation in A. niger. Here, we report the biochemical and functional characterization of these enzymes. AgnB is an α-1,3-glucanase that releases glucose from α-1,3-glucan substrates with a minimum degree of polymerization of 4. CfcA is a chitinase that releases dimers from the nonreducing end of chitin. These enzymes thus attack polymers that are found in the fungal cell wall and may have a role in autolytic fungal cell wall degradation in A. niger. Indeed, cell wall degradation during carbon starvation was reduced in the double deletion mutant ΔcfcA ΔagnB compared to the wild-type strain. Furthermore, the cell walls of the carbon-starved mycelium of the mutant contained a higher fraction of chitin or chitosan. The function of at least one of these enzymes, CfcA, therefore appears to be in the recycling of cell wall carbohydrates under carbon limiting conditions. CfcA thus may be a candidate effector for on demand cell lysis, which could be employed in industrial processes for recovery of intracellular products. Springer-Verlag Berlin Heidelberg, 2014 Aspergillus niger nationallicence Carbon starvation nationallicence Cell wall nationallicence Autolysis nationallicence Chitinase nationallicence α-Glucanase nationallicence van Munster Jolanda Microbial Physiology Research Group, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands aut Dobruchowska Justyna Microbial Physiology Research Group, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands aut Veloo Ruud Kerry BV, Almere, The Netherlands aut Dijkhuizen Lubbert Microbial Physiology Research Group, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands aut van der Maarel Marc Microbial Physiology Research Group, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2209-2223 0175-7598 99:5<2209 2015 99 253 https://doi.org/10.1007/s00253-014-6062-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6062-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Munster Jolanda Microbial Physiology Research Group, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands aut NATIONALLICENCE 700 1- Dobruchowska Justyna Microbial Physiology Research Group, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands aut NATIONALLICENCE 700 1- Veloo Ruud Kerry BV, Almere, The Netherlands aut NATIONALLICENCE 700 1- Dijkhuizen Lubbert Microbial Physiology Research Group, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands aut NATIONALLICENCE 700 1- van der Maarel Marc Microbial Physiology Research Group, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2209-2223 0175-7598 99:5<2209 2015 99 253