caa a22 4500 60550461X CHVBK 20210128100617.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6965-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6965-7 Lost signature: progress and failures in in vivo tracking of implanted stem cells [Elektronische Daten] [K. von der Haar, A. Lavrentieva, F. Stahl, T. Scheper, C. Blume] Stem cell therapy as a part of regenerative medicine provides promising approaches for the treatment of injuries and diseases. The increasing use of mesenchymal stem cells in various medical treatments created the demand for long-term in vivo cell tracking methods. Therefore, it is necessary to analyze post-transplantational survival, biodistribution, and engraftment of cells. Furthermore, stem cell treatment has been discussed controversially due to possible association with tumor formation in the recipient. For therapeutic purpose, stem cells must undergo substantial manipulation such as differentiation and in vitro expansion, and this can lead to the occurrence of genetic aberrations and altered expression of both tumor suppression and carcinogenic factors. To control therapy, it is necessary to find a reliable and general method to track and identify implanted cells in the recipient. This is especially challenging for autologous transplantations, as standard fingerprinting methods cannot be applied. An optimal technique for in vivo cell monitoring does not yet exist, and its development holds several challenges: small numbers of transplanted cells, possibility of cell number quantification, minimal transfer of the contrast agent to non-transplanted cells, and no genetic modification. This review discusses most of the proposed solutions, including magnetic resonance imaging, magnetic particle imaging, positron emission tomography, single-photon emission computed tomography, and optical imaging methods. Additionally, the recent research on cell labeling for stem cell monitoring after transplantation including in vitro, ex vivo, and in vivo imaging studies is described. Promising future imaging modalities for stem cell monitoring after transplantation are shown. Springer-Verlag Berlin Heidelberg, 2015 Stem cells nationallicence In vivo imaging nationallicence PET nationallicence SPECT nationallicence MRI nationallicence FLI nationallicence von der Haar K. Institute of Technical Chemistry, Leibniz University Hanover, Callinstrasse 5, 30167, Hannover, Germany aut Lavrentieva A. Institute of Technical Chemistry, Leibniz University Hanover, Callinstrasse 5, 30167, Hannover, Germany aut Stahl F. Institute of Technical Chemistry, Leibniz University Hanover, Callinstrasse 5, 30167, Hannover, Germany aut Scheper T. Institute of Technical Chemistry, Leibniz University Hanover, Callinstrasse 5, 30167, Hannover, Germany aut Blume C. Institute of Technical Chemistry, Leibniz University Hanover, Callinstrasse 5, 30167, Hannover, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 9907-9922 0175-7598 99:23<9907 2015 99 253 https://doi.org/10.1007/s00253-015-6965-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6965-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- von der Haar K. Institute of Technical Chemistry, Leibniz University Hanover, Callinstrasse 5, 30167, Hannover, Germany aut NATIONALLICENCE 700 1- Lavrentieva A. Institute of Technical Chemistry, Leibniz University Hanover, Callinstrasse 5, 30167, Hannover, Germany aut NATIONALLICENCE 700 1- Stahl F. Institute of Technical Chemistry, Leibniz University Hanover, Callinstrasse 5, 30167, Hannover, Germany aut NATIONALLICENCE 700 1- Scheper T. Institute of Technical Chemistry, Leibniz University Hanover, Callinstrasse 5, 30167, Hannover, Germany aut NATIONALLICENCE 700 1- Blume C. Institute of Technical Chemistry, Leibniz University Hanover, Callinstrasse 5, 30167, Hannover, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 9907-9922 0175-7598 99:23<9907 2015 99 253