caa a22 4500 605504628 CHVBK 20210128100617.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6892-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6892-7 DasR is a pleiotropic regulator required for antibiotic production, pigment biosynthesis, and morphological development in Saccharopolyspora erythraea [Elektronische Daten] [Cheng-Heng Liao, Ya Xu, Sébastien Rigali, Bang-Ce Ye] The GntR-family transcription regulator, DasR, was previously identified as pleiotropic, controlling the primary amino sugar N-acetylglucosamine (GlcNAc) and chitin metabolism in Saccharopolyspora erythraea and Streptomyces coelicolor. Due to the remarkable regulatory impact of DasR on antibiotic production and development in the model strain of S. coelicolor, we here identified and characterized the role of DasR to secondary metabolite production and morphological development in industrial erythromycin-producing S. erythraea. The physiological studies have shown that a constructed deletion of dasR in S. erythraea resulted in antibiotic, pigment, and aerial hyphae production deficit in a nutrient-rich condition. DNA microarray assay, combined with quantitative real-time reverse transcription PCR (qRT-PCR), confirmed these results by showing the downregulation of the genes relating to secondary metabolite production in the dasR null mutant. Notably, electrophoretic mobility shift assays (EMSA) showed DasR as being the first identified regulator that directly regulates the pigment biosynthesis rpp gene cluster. In addition, further studies indicated that GlcNAc, the major nutrient signal of DasR-responsed regulation, blocked secondary metabolite production and morphological development. The effects of GlcNAc were shown to be caused by DasR mediation. These findings demonstrated that DasR is an important pleiotropic regulator for both secondary metabolism and morphological development in S. erythraea, providing new insights for the genetic engineering of S. erythraea with increased erythromycin production. Springer-Verlag Berlin Heidelberg, 2015 Saccharopolyspora erythraea nationallicence DasR nationallicence Secondary metabolism nationallicence Morphological development nationallicence Liao Cheng-Heng Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China aut Xu Ya Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China aut Rigali Sébastien Centre for Protein Engineering, Institut de Chimie B6a, University of Liège, 4000, Liège, Belgium aut Ye Bang-Ce Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 10215-10224 0175-7598 99:23<10215 2015 99 253 https://doi.org/10.1007/s00253-015-6892-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6892-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liao Cheng-Heng Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China aut NATIONALLICENCE 700 1- Xu Ya Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China aut NATIONALLICENCE 700 1- Rigali Sébastien Centre for Protein Engineering, Institut de Chimie B6a, University of Liège, 4000, Liège, Belgium aut NATIONALLICENCE 700 1- Ye Bang-Ce Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 10215-10224 0175-7598 99:23<10215 2015 99 253