Towards an effective biosensor for monitoring AD leachate: a knockout E. coli mutant that cannot catabolise lactate
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6887-4 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6887-4 | ||
| 245 | 0 | 0 | |a Towards an effective biosensor for monitoring AD leachate: a knockout E. coli mutant that cannot catabolise lactate |h [Elektronische Daten] |c [Joseph Sweeney, Cormac Murphy, Kevin McDonnell] |
| 520 | 3 | |a Development of a biosensor for the convenient measurement of acetate and propionate concentrations in a two-phase anaerobic digestor (AD) requires a bacterium that will be unresponsive to the other organic acids present in the leachate, of which lactate is the most abundant. Successive gene knockouts of E.coli W3110 d-lactate dehydrogenase (dld), l-lactate dehydrogenase (lldD), glycolate oxidase (glcD) and a suspected l-lactate dehdrogenase (ykgF) were performed. The resulting quadruple mutant (IMD Wldgy) was incapable of growth on d- and l-lactate, whereas the wild type grew readily on these substrates. Furthermore, the O2 consumption rates of acetate-grown IMD Wldgy cell suspensions supplied with either acetate (0.1mM) or a synthetic leachate including acetate (0.1mM) and dl-lactate (1mM) were identical (2.79 and 2.70mgl−1min−1, respectively). This was in marked contrast to similar experiments with the wild type which gave initial O2 consumption rates of 2.00, 2.36 and 2.97mgl−1min−1 when cell suspensions were supplied with acetate (0.1mM), acetate (0.1mM) plus d-lactate (1mM) or acetate (0.1mM) plus l-lactate (1mM), respectively. The knockout strain provides a platform for the design of a biosensor that can accessibly monitor acetate and propionate concentrations in AD leachate via O2-uptake measurements. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Anaerobic digestion |2 nationallicence | |
| 690 | 7 | |a Biosensor |2 nationallicence | |
| 690 | 7 | |a Volatile fatty acids |2 nationallicence | |
| 690 | 7 | |a Synthetic leachate |2 nationallicence | |
| 700 | 1 | |a Sweeney |D Joseph |u UCD Schools of Biosystems Engineering, Food and Agriculture, University College Dublin, Belfield, Dublin 4, Ireland |4 aut | |
| 700 | 1 | |a Murphy |D Cormac |u UCD School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland |4 aut | |
| 700 | 1 | |a McDonnell |D Kevin |u UCD Schools of Biosystems Engineering, Food and Agriculture, University College Dublin, Belfield, Dublin 4, Ireland |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/23(2015-12-01), 10209-10214 |x 0175-7598 |q 99:23<10209 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6887-4 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6887-4 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sweeney |D Joseph |u UCD Schools of Biosystems Engineering, Food and Agriculture, University College Dublin, Belfield, Dublin 4, Ireland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Murphy |D Cormac |u UCD School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a McDonnell |D Kevin |u UCD Schools of Biosystems Engineering, Food and Agriculture, University College Dublin, Belfield, Dublin 4, Ireland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/23(2015-12-01), 10209-10214 |x 0175-7598 |q 99:23<10209 |1 2015 |2 99 |o 253 | ||