Direct quantification of fatty acids in wet microalgal and yeast biomass via a rapid in situ fatty acid methyl ester derivatization approach
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| 008 | 210128e20151201xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6909-2 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6909-2 | ||
| 245 | 0 | 0 | |a Direct quantification of fatty acids in wet microalgal and yeast biomass via a rapid in situ fatty acid methyl ester derivatization approach |h [Elektronische Daten] |c [Tao Dong, Liang Yu, Difeng Gao, Xiaochen Yu, Chao Miao, Yubin Zheng, Jieni Lian, Tingting Li, Shulin Chen] |
| 520 | 3 | |a Accurate determination of fatty acid contents is routinely required in microalgal and yeast biofuel studies. A method of rapid in situ fatty acid methyl ester (FAME) derivatization directly from wet fresh microalgal and yeast biomass was developed in this study. This method does not require prior solvent extraction or dehydration. FAMEs were prepared with a sequential alkaline hydrolysis (15min at 85°C) and acidic esterification (15min at 85°C) process. The resulting FAMEs were extracted into n-hexane and analyzed using gas chromatography. The effects of each processing parameter (temperature, reaction time, and water content) upon the lipids quantification in the alkaline hydrolysis step were evaluated with a full factorial design. This method could tolerate water content up to 20% (v/v) in total reaction volume, which equaled up to 1.2mL of water in biomass slurry (with 0.05-25mg of fatty acid). There were no significant differences in FAME quantification (p>0.05) between the standard AOAC 991.39 method and the proposed wet in situ FAME preparation method. This fatty acid quantification method is applicable to fresh wet biomass of a wide range of microalgae and yeast species. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Biofuel |2 nationallicence | |
| 690 | 7 | |a Microalgae |2 nationallicence | |
| 690 | 7 | |a Yeast |2 nationallicence | |
| 690 | 7 | |a GC analysis |2 nationallicence | |
| 690 | 7 | |a FAME |2 nationallicence | |
| 690 | 7 | |a Esterification |2 nationallicence | |
| 700 | 1 | |a Dong |D Tao |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | |
| 700 | 1 | |a Yu |D Liang |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | |
| 700 | 1 | |a Gao |D Difeng |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | |
| 700 | 1 | |a Yu |D Xiaochen |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | |
| 700 | 1 | |a Miao |D Chao |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | |
| 700 | 1 | |a Zheng |D Yubin |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | |
| 700 | 1 | |a Lian |D Jieni |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | |
| 700 | 1 | |a Li |D Tingting |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | |
| 700 | 1 | |a Chen |D Shulin |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/23(2015-12-01), 10237-10247 |x 0175-7598 |q 99:23<10237 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6909-2 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6909-2 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dong |D Tao |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Liang |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gao |D Difeng |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Xiaochen |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Miao |D Chao |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zheng |D Yubin |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lian |D Jieni |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Tingting |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chen |D Shulin |u Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/23(2015-12-01), 10237-10247 |x 0175-7598 |q 99:23<10237 |1 2015 |2 99 |o 253 | ||