caa a22 4500 60550475X CHVBK 20210128100618.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6909-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6909-2 Direct quantification of fatty acids in wet microalgal and yeast biomass via a rapid in situ fatty acid methyl ester derivatization approach [Elektronische Daten] [Tao Dong, Liang Yu, Difeng Gao, Xiaochen Yu, Chao Miao, Yubin Zheng, Jieni Lian, Tingting Li, Shulin Chen] Accurate determination of fatty acid contents is routinely required in microalgal and yeast biofuel studies. A method of rapid in situ fatty acid methyl ester (FAME) derivatization directly from wet fresh microalgal and yeast biomass was developed in this study. This method does not require prior solvent extraction or dehydration. FAMEs were prepared with a sequential alkaline hydrolysis (15min at 85°C) and acidic esterification (15min at 85°C) process. The resulting FAMEs were extracted into n-hexane and analyzed using gas chromatography. The effects of each processing parameter (temperature, reaction time, and water content) upon the lipids quantification in the alkaline hydrolysis step were evaluated with a full factorial design. This method could tolerate water content up to 20% (v/v) in total reaction volume, which equaled up to 1.2mL of water in biomass slurry (with 0.05-25mg of fatty acid). There were no significant differences in FAME quantification (p>0.05) between the standard AOAC 991.39 method and the proposed wet in situ FAME preparation method. This fatty acid quantification method is applicable to fresh wet biomass of a wide range of microalgae and yeast species. Springer-Verlag Berlin Heidelberg, 2015 Biofuel nationallicence Microalgae nationallicence Yeast nationallicence GC analysis nationallicence FAME nationallicence Esterification nationallicence Dong Tao Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut Yu Liang Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut Gao Difeng Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut Yu Xiaochen Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut Miao Chao Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut Zheng Yubin Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut Lian Jieni Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut Li Tingting Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut Chen Shulin Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 10237-10247 0175-7598 99:23<10237 2015 99 253 https://doi.org/10.1007/s00253-015-6909-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6909-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dong Tao Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut NATIONALLICENCE 700 1- Yu Liang Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut NATIONALLICENCE 700 1- Gao Difeng Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut NATIONALLICENCE 700 1- Yu Xiaochen Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut NATIONALLICENCE 700 1- Miao Chao Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut NATIONALLICENCE 700 1- Zheng Yubin Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut NATIONALLICENCE 700 1- Lian Jieni Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut NATIONALLICENCE 700 1- Li Tingting Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut NATIONALLICENCE 700 1- Chen Shulin Department of Biological Systems Engineering, Washington State University, 99164-6120, Pullman, WA, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 10237-10247 0175-7598 99:23<10237 2015 99 253