caa a22 4500 605504814 CHVBK 20210128100618.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6896-3 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6896-3 Photosynthetic aeration in biological wastewater treatment using immobilized microalgae-bacteria symbiosis [Elektronische Daten] [Prashant Praveen, Kai-Chee Loh] Chlorella vulgaris encapsulated in alginate beads were added into a bioreactor treating synthetic wastewater using Pseudomonas putida. A symbiotic CO2/O2 gas exchange was established between the two microorganisms for photosynthetic aeration of wastewater. During batch operation, glucose removal efficiency in the bioreactor improved from 50% in 12h without aeration to 100% in 6h, when the bioreactor was aerated photosynthetically. During continuous operation, the bioreactor was operated at a low hydraulic retention time of 3.3h at feed concentrations of 250 and 500mg/L glucose. The removal efficiency at 500mg/L increased from 73% without aeration to 100% in the presence of immobilized microalgae. The initial microalgae concentration was critical to achieve adequate aeration, and the removal rate increased with increasing microalgae concentration. The highest removal rate of 142mg/L-h glucose was achieved at an initial microalgae concentration of 190mg/L. Quantification of microalgae growth in the alginate beads indicated an exponential growth during symbiosis, indicating that the bioreactor performance was limited by oxygen production rates. Under symbiotic conditions, the chlorophyll content of the immobilized microalgae increased by more than 30%. These results indicate that immobilized microalgae in symbiosis with heterotrophic bacteria are promising in wastewater aeration. Springer-Verlag Berlin Heidelberg, 2015 Aeration nationallicence Biodegradation nationallicence Cell immobilization nationallicence Microalgae nationallicence Symbiosis nationallicence Praveen Prashant Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, 117585, Singapore, Singapore aut Loh Kai-Chee Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, 117585, Singapore, Singapore aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 10345-10354 0175-7598 99:23<10345 2015 99 253 https://doi.org/10.1007/s00253-015-6896-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6896-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Praveen Prashant Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, 117585, Singapore, Singapore aut NATIONALLICENCE 700 1- Loh Kai-Chee Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, 117585, Singapore, Singapore aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 10345-10354 0175-7598 99:23<10345 2015 99 253