Identification and biochemical characterization of a novel endo-type β-agarase AgaW from Cohnella sp. strain LGH
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6869-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6869-6 | ||
| 245 | 0 | 0 | |a Identification and biochemical characterization of a novel endo-type β-agarase AgaW from Cohnella sp. strain LGH |h [Elektronische Daten] |c [Gen Li, Mingming Sun, Jun Wu, Mao Ye, Xincheng Ge, Wei Wei, Huixin Li, Feng Hu] |
| 520 | 3 | |a An agar-degrading bacterium, strain LGH, was isolated and identified as Cohnella sp. This strain had a capability of utilizing agar as a sole carbon source for growth and showed a strong agarolytic activity. A novel endo-type β-agarase gene agaW, encoding a primary translation product of 891 amino acids, including a 26 amino acid signal peptide, was cloned and identified from a genomic library of strain LGH. The AgaW belonged to the glycoside hydrolase (GH) GH50 family, with less than 39% amino acid sequence similarity with any known protein, and hydrolyzed agarose into neoagarotetraose as the major end product and neoagarobiose as the minor end product through other neoagarooligosaccharide intermediates, such as neoagarohexaose. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Cohnella sp. LGH |2 nationallicence | |
| 690 | 7 | |a Endo-type β-agarase |2 nationallicence | |
| 690 | 7 | |a Neoagarotetraose |2 nationallicence | |
| 690 | 7 | |a Neoagarobiose |2 nationallicence | |
| 700 | 1 | |a Li |D Gen |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | |
| 700 | 1 | |a Sun |D Mingming |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | |
| 700 | 1 | |a Wu |D Jun |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | |
| 700 | 1 | |a Ye |D Mao |u State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, People's Republic of China |4 aut | |
| 700 | 1 | |a Ge |D Xincheng |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | |
| 700 | 1 | |a Wei |D Wei |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | |
| 700 | 1 | |a Li |D Huixin |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | |
| 700 | 1 | |a Hu |D Feng |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/23(2015-12-01), 10019-10029 |x 0175-7598 |q 99:23<10019 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6869-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6869-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Gen |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sun |D Mingming |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wu |D Jun |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ye |D Mao |u State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ge |D Xincheng |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wei |D Wei |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Huixin |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hu |D Feng |u Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/23(2015-12-01), 10019-10029 |x 0175-7598 |q 99:23<10019 |1 2015 |2 99 |o 253 | ||