caa a22 4500 605504849 CHVBK 20210128100618.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6869-6 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6869-6 Identification and biochemical characterization of a novel endo-type β-agarase AgaW from Cohnella sp. strain LGH [Elektronische Daten] [Gen Li, Mingming Sun, Jun Wu, Mao Ye, Xincheng Ge, Wei Wei, Huixin Li, Feng Hu] An agar-degrading bacterium, strain LGH, was isolated and identified as Cohnella sp. This strain had a capability of utilizing agar as a sole carbon source for growth and showed a strong agarolytic activity. A novel endo-type β-agarase gene agaW, encoding a primary translation product of 891 amino acids, including a 26 amino acid signal peptide, was cloned and identified from a genomic library of strain LGH. The AgaW belonged to the glycoside hydrolase (GH) GH50 family, with less than 39% amino acid sequence similarity with any known protein, and hydrolyzed agarose into neoagarotetraose as the major end product and neoagarobiose as the minor end product through other neoagarooligosaccharide intermediates, such as neoagarohexaose. Springer-Verlag Berlin Heidelberg, 2015 Cohnella sp. LGH nationallicence Endo-type β-agarase nationallicence Neoagarotetraose nationallicence Neoagarobiose nationallicence Li Gen Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Sun Mingming Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Wu Jun Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Ye Mao State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, People's Republic of China aut Ge Xincheng Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Wei Wei Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Li Huixin Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Hu Feng Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 10019-10029 0175-7598 99:23<10019 2015 99 253 https://doi.org/10.1007/s00253-015-6869-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6869-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Gen Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Sun Mingming Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Wu Jun Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Ye Mao State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Ge Xincheng Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Wei Wei Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Li Huixin Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Hu Feng Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 10019-10029 0175-7598 99:23<10019 2015 99 253