A novel enzyme-based antimicrobial system comprising iodide and a multicopper oxidase isolated from Alphaproteobacterium strain Q-1
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605504873 | ||
| 003 | CHVBK | ||
| 005 | 20210128100618.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20151201xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6862-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6862-0 | ||
| 245 | 0 | 2 | |a A novel enzyme-based antimicrobial system comprising iodide and a multicopper oxidase isolated from Alphaproteobacterium strain Q-1 |h [Elektronische Daten] |c [Tri Yuliana, Kyota Ebihara, Mio Suzuki, Chie Shimonaka, Seigo Amachi] |
| 520 | 3 | |a Alphaproteobacterium strain Q-1 produces an extracellular multicopper oxidase (IOX), which catalyzes iodide (I-) oxidation to form molecular iodine (I2). In this study, the antimicrobial activity of the IOX/iodide system was determined. Both Gram-positive and Gram-negative bacteria tested were killed completely within 5min by 50mUmL-1 of IOX and 10mM iodide. The sporicidal activity of the system was also tested and compared with a common iodophor, povidone-iodine (PVP-I). IOX (300mUmL-1) killed Bacillus cereus, B. subtilis, and Geobacillus stearothermophilus spores with decimal reduction times of 2.58, 7.62, and 40.9min, respectively. However, 0.1% PVP-I killed these spores with much longer decimal reduction times of 5.46, 38.0, and 260min, respectively. To evaluate the more superior sporicidal activity of the IOX system over PVP-I, the amount of free iodine (non-complexed I2) was determined by an equilibrium dialysis technique. The IOX system included more than 40mgL-1 of free iodine, while PVP-I included at most 25mgL-1 free iodine. Our results suggest that the new enzyme-based antimicrobial system is effective against a wide variety of microorganisms and bacterial spores, and that its strong biocidal activity is due to its high free iodine content, which is probably maintained by re-oxidation of iodide released after oxidation of cell components by I2. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Multicopper oxidase |2 nationallicence | |
| 690 | 7 | |a Iodide |2 nationallicence | |
| 690 | 7 | |a Antimicrobial system |2 nationallicence | |
| 690 | 7 | |a Sporicidal activity |2 nationallicence | |
| 690 | 7 | |a Povidone-iodine |2 nationallicence | |
| 700 | 1 | |a Yuliana |D Tri |u Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan |4 aut | |
| 700 | 1 | |a Ebihara |D Kyota |u Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan |4 aut | |
| 700 | 1 | |a Suzuki |D Mio |u Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan |4 aut | |
| 700 | 1 | |a Shimonaka |D Chie |u Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan |4 aut | |
| 700 | 1 | |a Amachi |D Seigo |u Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/23(2015-12-01), 10011-10018 |x 0175-7598 |q 99:23<10011 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6862-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6862-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yuliana |D Tri |u Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ebihara |D Kyota |u Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Suzuki |D Mio |u Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shimonaka |D Chie |u Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Amachi |D Seigo |u Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/23(2015-12-01), 10011-10018 |x 0175-7598 |q 99:23<10011 |1 2015 |2 99 |o 253 | ||