caa a22 4500 605504873 CHVBK 20210128100618.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6862-0 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6862-0 A novel enzyme-based antimicrobial system comprising iodide and a multicopper oxidase isolated from Alphaproteobacterium strain Q-1 [Elektronische Daten] [Tri Yuliana, Kyota Ebihara, Mio Suzuki, Chie Shimonaka, Seigo Amachi] Alphaproteobacterium strain Q-1 produces an extracellular multicopper oxidase (IOX), which catalyzes iodide (I-) oxidation to form molecular iodine (I2). In this study, the antimicrobial activity of the IOX/iodide system was determined. Both Gram-positive and Gram-negative bacteria tested were killed completely within 5min by 50mUmL-1 of IOX and 10mM iodide. The sporicidal activity of the system was also tested and compared with a common iodophor, povidone-iodine (PVP-I). IOX (300mUmL-1) killed Bacillus cereus, B. subtilis, and Geobacillus stearothermophilus spores with decimal reduction times of 2.58, 7.62, and 40.9min, respectively. However, 0.1% PVP-I killed these spores with much longer decimal reduction times of 5.46, 38.0, and 260min, respectively. To evaluate the more superior sporicidal activity of the IOX system over PVP-I, the amount of free iodine (non-complexed I2) was determined by an equilibrium dialysis technique. The IOX system included more than 40mgL-1 of free iodine, while PVP-I included at most 25mgL-1 free iodine. Our results suggest that the new enzyme-based antimicrobial system is effective against a wide variety of microorganisms and bacterial spores, and that its strong biocidal activity is due to its high free iodine content, which is probably maintained by re-oxidation of iodide released after oxidation of cell components by I2. Springer-Verlag Berlin Heidelberg, 2015 Multicopper oxidase nationallicence Iodide nationallicence Antimicrobial system nationallicence Sporicidal activity nationallicence Povidone-iodine nationallicence Yuliana Tri Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut Ebihara Kyota Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut Suzuki Mio Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut Shimonaka Chie Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut Amachi Seigo Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 10011-10018 0175-7598 99:23<10011 2015 99 253 https://doi.org/10.1007/s00253-015-6862-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6862-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yuliana Tri Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut NATIONALLICENCE 700 1- Ebihara Kyota Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut NATIONALLICENCE 700 1- Suzuki Mio Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut NATIONALLICENCE 700 1- Shimonaka Chie Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut NATIONALLICENCE 700 1- Amachi Seigo Graduate School of Horticulture, Chiba University, 648 Matsudo, 271-8510, Matsudo City, Chiba, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 10011-10018 0175-7598 99:23<10011 2015 99 253