caa a22 4500 605504970 CHVBK 20210128100619.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00253-015-6894-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6894-5 Two-step nitrification in a pure moving bed biofilm reactor-membrane bioreactor for wastewater treatment: nitrifying and denitrifying microbial populations and kinetic modeling [Elektronische Daten] [J. Leyva-Díaz, A. González-Martínez, M. Muñío, J. Poyatos] The moving bed biofilm reactor-membrane bioreactor (MBBR-MBR) is a novel solution to conventional activated sludge processes and membrane bioreactors. In this study, a pure MBBR-MBR was studied. The pure MBBR-MBR mainly had attached biomass. The bioreactor operated with a hydraulic retention time (HRT) of 9.5h. The kinetic parameters for heterotrophic and autotrophic biomasses, mainly nitrite-oxidizing bacteria (NOB), were evaluated. The analysis of the bacterial community structure of the ammonium-oxidizing bacteria (AOB), NOB, and denitrifying bacteria (DeNB) from the pure MBBR-MBR was carried out by means of pyrosequencing to detect and quantify the contribution of the nitrifying and denitrifying bacteria in the total bacterial community. The relative abundance of AOB, NOB, and DeNB were 5, 1, and 3%, respectively, in the mixed liquor suspended solids (MLSS), and these percentages were 18, 5, and 2%, respectively, in the biofilm density (BD) attached to carriers. The pure MBBR-MBR had a high efficiency of total nitrogen (TN) removal of 71.81±16.04%, which could reside in the different bacterial assemblages in the fixed biofilm on the carriers. In this regard, the kinetic parameters for autotrophic biomass had values of Y A =2.3465mgO2mgN−1, μ m, A=0.7169h−1, and K NH=2.0748mgNL−1. Springer-Verlag Berlin Heidelberg, 2015 Wastewater treatment nationallicence Moving bed biofilm reactor nationallicence Biofilm nationallicence Two-step nitrification nationallicence Pyrosequencing nationallicence Autotrophic kinetics nationallicence Leyva-Díaz J. Department of Civil Engineering, University of Granada, 18071, Granada, Spain aut González-Martínez A. Department of Civil Engineering, University of Granada, 18071, Granada, Spain aut Muñío M. Department of Chemical Engineering, University of Granada, 18071, Granada, Spain aut Poyatos J. Department of Civil Engineering, University of Granada, 18071, Granada, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 10333-10343 0175-7598 99:23<10333 2015 99 253 https://doi.org/10.1007/s00253-015-6894-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6894-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leyva-Díaz J. Department of Civil Engineering, University of Granada, 18071, Granada, Spain aut NATIONALLICENCE 700 1- González-Martínez A. Department of Civil Engineering, University of Granada, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Muñío M. Department of Chemical Engineering, University of Granada, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Poyatos J. Department of Civil Engineering, University of Granada, 18071, Granada, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/23(2015-12-01), 10333-10343 0175-7598 99:23<10333 2015 99 253