caa a22 4500 605504997 CHVBK 20210128100619.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6037-4 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6037-4 Methods for visualising active microbial benzene degraders in in situ microcosms [Elektronische Daten] [Christian Schurig, Carsten Mueller, Carmen Höschen, Andrea Prager, Erika Kothe, Henrike Beck, Anja Miltner, Matthias Kästner] Natural attenuation maybe a cost-efficient option for bioremediation of contaminated sites but requires knowledge about the activity of degrading microbes under in situ conditions. In order to link microbial activity to the spatial distribution of contaminant degraders, we combined the recently improved in situ microcosm approach, so-called ‘direct-push bacterial trap' (DP-BACTRAP), with nano-scale secondary ion mass spectrometry (NanoSIMS) analysis on samples from contaminated constructed wetlands. This approach is based on initially sterile microcosms amended with 13C-labelled benzene as a source of carbon and energy for microorganisms. The microcosms were introduced directly in the constructed wetland, where they were colonised by indigenous microorganisms from the sediment. After incubation in the field, the samples were analysed by NanoSIMS, scanning electron microscopy (SEM) and fluorescence microscopy in order to visualise 13C-labelled microbial biomass on undisturbed samples from the microcosms. With the approach developed, we successfully visualised benzene-degrading microbes on solid materials with high surface area by means of NanoSIMS. Moreover, we could demonstrate the feasibility of NanoSIMS analysis of unembedded porous media with a highly complex topography, which was frequently reasoned to not lead to sufficient results. Springer-Verlag Berlin Heidelberg, 2014 NanoSIMS nationallicence 13C nationallicence Microbial activity nationallicence In situ microcosms nationallicence DP-BACTRAPs nationallicence Benzene nationallicence Schurig Christian Department for Environmental Biotechnology, Helmholtz Centre for Environmental Research—UFZ, Leipzig, Germany aut Mueller Carsten Lehrstuhl für Bodenkunde, Technische Universität München, Emil-Ramann-Str. 2, 85354, Freising-Weihenstephan, Germany aut Höschen Carmen Lehrstuhl für Bodenkunde, Technische Universität München, Emil-Ramann-Str. 2, 85354, Freising-Weihenstephan, Germany aut Prager Andrea Leibnitz Institut für Oberflächenmodifizierung, Leipzig, Germany aut Kothe Erika Microbial Communication, Institute of Microbiology, Faculty for Biology and Pharmacy, Friedrich Schiller University of Jena, Jena, Germany aut Beck Henrike Department for Environmental Biotechnology, Helmholtz Centre for Environmental Research—UFZ, Leipzig, Germany aut Miltner Anja Department for Environmental Biotechnology, Helmholtz Centre for Environmental Research—UFZ, Leipzig, Germany aut Kästner Matthias Department for Environmental Biotechnology, Helmholtz Centre for Environmental Research—UFZ, Leipzig, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 957-968 0175-7598 99:2<957 2015 99 253 https://doi.org/10.1007/s00253-014-6037-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6037-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schurig Christian Department for Environmental Biotechnology, Helmholtz Centre for Environmental Research—UFZ, Leipzig, Germany aut NATIONALLICENCE 700 1- Mueller Carsten Lehrstuhl für Bodenkunde, Technische Universität München, Emil-Ramann-Str. 2, 85354, Freising-Weihenstephan, Germany aut NATIONALLICENCE 700 1- Höschen Carmen Lehrstuhl für Bodenkunde, Technische Universität München, Emil-Ramann-Str. 2, 85354, Freising-Weihenstephan, Germany aut NATIONALLICENCE 700 1- Prager Andrea Leibnitz Institut für Oberflächenmodifizierung, Leipzig, Germany aut NATIONALLICENCE 700 1- Kothe Erika Microbial Communication, Institute of Microbiology, Faculty for Biology and Pharmacy, Friedrich Schiller University of Jena, Jena, Germany aut NATIONALLICENCE 700 1- Beck Henrike Department for Environmental Biotechnology, Helmholtz Centre for Environmental Research—UFZ, Leipzig, Germany aut NATIONALLICENCE 700 1- Miltner Anja Department for Environmental Biotechnology, Helmholtz Centre for Environmental Research—UFZ, Leipzig, Germany aut NATIONALLICENCE 700 1- Kästner Matthias Department for Environmental Biotechnology, Helmholtz Centre for Environmental Research—UFZ, Leipzig, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 957-968 0175-7598 99:2<957 2015 99 253