caa a22 4500 605505004 CHVBK 20210128100619.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6215-4 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6215-4 Overview on the biotechnological production of l -DOPA [Elektronische Daten] [Kyoungseon Min, Kyungmoon Park, Don-Hee Park, Young Yoo] l-DOPA (3,4-dihydroxyphenyl-l-alanine) has been widely used as a drug for Parkinson's disease caused by deficiency of the neurotransmitter dopamine. Since Monsanto developed the commercial process for l-DOPA synthesis for the first time, most of currently supplied l-DOPA has been produced by the asymmetric method, especially asymmetric hydrogenation. However, the asymmetric synthesis shows critical limitations such as a poor conversion rate and a low enantioselectivity. Accordingly, alternative biotechnological approaches have been researched for overcoming the shortcomings: microbial fermentation using microorganisms with tyrosinase, tyrosine phenol-lyase, or p-hydroxyphenylacetate 3-hydroxylase activity and enzymatic conversion by immobilized tyrosinase. Actually, Ajinomoto Co. Ltd commercialized Erwinia herbicola fermentation to produce l-DOPA from catechol. In addition, the electroenzymatic conversion system was recently introduced as a newly emerging scheme. In this review, we aim to not only overview the biotechnological l-DOPA production methods, but also to briefly compare and analyze their advantages and drawbacks. Furthermore, we suggest the future potential of biotechnological l-DOPA production as an industrial process. Springer-Verlag Berlin Heidelberg, 2014 l -DOPA nationallicence Microbial fermentation nationallicence Immobilized tyrosinase nationallicence Electroenzymatic system nationallicence Min Kyoungseon Clean Energy Research Center, Korea Institute of Science and Technology, 136-791, Seoul, South Korea aut Park Kyungmoon Department of Biological and Chemical Engineering, Hongik University, 339-701, Sejong, South Korea aut Park Don-Hee Department of Biotechnology and Bioengineering, Chonnam National University, 500-751, Gwangju, South Korea aut Yoo Young School of Chemical and Biological Engineering, Seoul National University, 151-742, Seoul, South Korea aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 575-584 0175-7598 99:2<575 2015 99 253 https://doi.org/10.1007/s00253-014-6215-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6215-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Min Kyoungseon Clean Energy Research Center, Korea Institute of Science and Technology, 136-791, Seoul, South Korea aut NATIONALLICENCE 700 1- Park Kyungmoon Department of Biological and Chemical Engineering, Hongik University, 339-701, Sejong, South Korea aut NATIONALLICENCE 700 1- Park Don-Hee Department of Biotechnology and Bioengineering, Chonnam National University, 500-751, Gwangju, South Korea aut NATIONALLICENCE 700 1- Yoo Young School of Chemical and Biological Engineering, Seoul National University, 151-742, Seoul, South Korea aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 575-584 0175-7598 99:2<575 2015 99 253