Methylotrophy in the thermophilic Bacillus methanolicus , basic insights and application for commodity production from methanol
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6224-3 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6224-3 | ||
| 245 | 0 | 0 | |a Methylotrophy in the thermophilic Bacillus methanolicus , basic insights and application for commodity production from methanol |h [Elektronische Daten] |c [Jonas Müller, Tonje Heggeset, Volker Wendisch, Julia Vorholt, Trygve Brautaset] |
| 520 | 3 | |a Using methanol as an alternative non-food feedstock for biotechnological production offers several advantages in line with a methanol-based bioeconomy. The Gram-positive, facultative methylotrophic and thermophilic bacterium Bacillus methanolicus is one of the few described microbial candidates with a potential for the conversion of methanol to value-added products. Its capabilities of producing and secreting the commercially important amino acids l-glutamate and l-lysine to high concentrations at 50°C have been demonstrated and make B. methanolicus a promising target to develop cell factories for industrial-scale production processes. B. methanolicus uses the ribulose monophosphate cycle for methanol assimilation and represents the first example of plasmid-dependent methylotrophy. Recent genome sequencing of two physiologically different wild-type B. methanolicus strains, MGA3 and PB1, accompanied with transcriptome and proteome analyses has generated fundamental new insight into the metabolism of the species. In addition, multiple key enzymes representing methylotrophic and biosynthetic pathways have been biochemically characterized. All this, together with establishment of improved tools for gene expression, has opened opportunities for systems-level metabolic engineering of B. methanolicus. Here, we summarize the current status of its metabolism and biochemistry, available genetic tools, and its potential use in respect to overproduction of amino acids. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Methylotrophy |2 nationallicence | |
| 690 | 7 | |a Ribulose monophosphate cycle |2 nationallicence | |
| 690 | 7 | |a l -Lysine biosynthesis |2 nationallicence | |
| 690 | 7 | |a l -Glutamate biosynthesis |2 nationallicence | |
| 690 | 7 | |a Genetic tools |2 nationallicence | |
| 690 | 7 | |a Industrial biotechnology |2 nationallicence | |
| 700 | 1 | |a Müller |D Jonas |u Institute of Microbiology, ETH Zurich, Zurich, Switzerland |4 aut | |
| 700 | 1 | |a Heggeset |D Tonje |u Department of Molecular Biology, SINTEF Materials and Chemistry, Sluppen, Postboks 4760, 7465, Trondheim, Norway |4 aut | |
| 700 | 1 | |a Wendisch |D Volker |u Chair of Genetics of Prokaryotes, Faculty of Biology and CeBiTec, Bielefeld University, Bielefeld, Germany |4 aut | |
| 700 | 1 | |a Vorholt |D Julia |u Institute of Microbiology, ETH Zurich, Zurich, Switzerland |4 aut | |
| 700 | 1 | |a Brautaset |D Trygve |u Department of Molecular Biology, SINTEF Materials and Chemistry, Sluppen, Postboks 4760, 7465, Trondheim, Norway |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 535-551 |x 0175-7598 |q 99:2<535 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6224-3 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a review-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6224-3 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Müller |D Jonas |u Institute of Microbiology, ETH Zurich, Zurich, Switzerland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Heggeset |D Tonje |u Department of Molecular Biology, SINTEF Materials and Chemistry, Sluppen, Postboks 4760, 7465, Trondheim, Norway |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wendisch |D Volker |u Chair of Genetics of Prokaryotes, Faculty of Biology and CeBiTec, Bielefeld University, Bielefeld, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Vorholt |D Julia |u Institute of Microbiology, ETH Zurich, Zurich, Switzerland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Brautaset |D Trygve |u Department of Molecular Biology, SINTEF Materials and Chemistry, Sluppen, Postboks 4760, 7465, Trondheim, Norway |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 535-551 |x 0175-7598 |q 99:2<535 |1 2015 |2 99 |o 253 | ||