caa a22 4500 605505071 CHVBK 20210128100620.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6036-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6036-5 Comparison of the microbial communities in solid-state anaerobic digestion (SS-AD) reactors operated at mesophilic and thermophilic temperatures [Elektronische Daten] [Yueh-Fen Li, Michael Nelson, Po-Hsu Chen, Joerg Graf, Yebo Li, Zhongtang Yu] The microbiomes involved in liquid anaerobic digestion process have been investigated extensively, but the microbiomes underpinning solid-state anaerobic digestion (SS-AD) are poorly understood. In this study, microbiome composition and temporal succession in batch SS-AD reactors, operated at mesophilic or thermophilic temperatures, were investigated using Illumina sequencing of 16S rRNA gene amplicons. A greater microbial richness and evenness were found in the mesophilic than in the thermophilic SS-AD reactors. Firmicutes accounted for 60 and 82% of the total Bacteria in the mesophilic and in the thermophilic SS-AD reactors, respectively. The genus Methanothermobacter dominated the Archaea in the thermophilic SS-AD reactors, while Methanoculleus predominated in the mesophilic SS-AD reactors. Interestingly, the data suggest syntrophic acetate oxidation coupled with hydrogenotrophic methanogenesis as an important pathway for biogas production during the thermophilic SS-AD. Canonical correspondence analysis (CCA)showed that temperature was the most influential factor in shaping the microbiomes in the SS-AD reactors. Thermotogae showed strong positive correlation with operation temperature, while Fibrobacteres, Lentisphaerae, Spirochaetes, and Tenericutes were positively correlated with daily biogas yield. This study provided new insight into the microbiome that drives SS-AD process, and the findings may help advance understanding of the microbiome in SS-AD reactors and the design and operation of SS-AD systems. Springer-Verlag Berlin Heidelberg, 2014 Illumina sequencing nationallicence 16S rRNA gene nationallicence Microbiome nationallicence Solid-state anaerobic digester nationallicence Li Yueh-Fen Environmental Science Graduate Program, The Ohio State University, 43210, Columbus, OH, USA aut Nelson Michael Department of Molecular and Cell Biology, University of Connecticut, 06269, Storrs, CT, USA aut Chen Po-Hsu Department of Statistics, The Ohio State University, 43210, Columbus, OH, USA aut Graf Joerg Department of Molecular and Cell Biology, University of Connecticut, 06269, Storrs, CT, USA aut Li Yebo Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave, 44691, Wooster, OH, USA aut Yu Zhongtang Environmental Science Graduate Program, The Ohio State University, 43210, Columbus, OH, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 969-980 0175-7598 99:2<969 2015 99 253 https://doi.org/10.1007/s00253-014-6036-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6036-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Yueh-Fen Environmental Science Graduate Program, The Ohio State University, 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Nelson Michael Department of Molecular and Cell Biology, University of Connecticut, 06269, Storrs, CT, USA aut NATIONALLICENCE 700 1- Chen Po-Hsu Department of Statistics, The Ohio State University, 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Graf Joerg Department of Molecular and Cell Biology, University of Connecticut, 06269, Storrs, CT, USA aut NATIONALLICENCE 700 1- Li Yebo Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave, 44691, Wooster, OH, USA aut NATIONALLICENCE 700 1- Yu Zhongtang Environmental Science Graduate Program, The Ohio State University, 43210, Columbus, OH, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 969-980 0175-7598 99:2<969 2015 99 253