caa a22 4500 605505098 CHVBK 20210128100620.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6048-1 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6048-1 Development of new tolerant strains to hydrophilic and hydrophobic organic solvents by the yeast surface display methodology [Elektronische Daten] [C. Perpiñá, J. Vinaixa, C. Andreu, M. del Olmo] Yeast surface display is a research methodology based on anchoring functional proteins and peptides onto the surface of the cells of this eukaryotic organism. Its development has resulted in the construction of a good number of new whole-cell biocatalysts with diverse applications in biotechnology, pharmacy, and medicine. In this work, we describe the design of new yeast strains in which several proteins and peptides have been introduced at the N-terminal position of protein agglutinin Aga2p. In all cases, proteins were correctly expressed and displayed on the cell surface according to the western blot, fluorescence microscopy, and fluorescence-activated cell sorting (FACS) analyses. The introduction of a glycosylable, Ser/Thr-rich protein (S1) resulted in improved resistance to ethanol, nonane, and dimethyl sulfoxide (DMSO) stress. The protein with a very high hydrophobic content (S2d) proved to confer tolerance to acetonitrile, ethanol, nonane, salt, and sodium dodecyl sulfate (SDS). The introduction of five leucine residues at the N-terminal position of S1 and S2 resulted in similar or increased resistance to the above-mentioned stress conditions. The adverse effects described in a previous work, when these residues were introduced into the N-terminus of Aga2p, with no other protein acting as a spacer, were not observed. Indeed, these strains grew better in the presence of hydrophilic solvents such as acetonitrile and ethanol. The new strains reported in this work have biotechnological potentiality given their behavior under adverse conditions of interest for biocatalytic and industrial processes. Springer-Verlag Berlin Heidelberg, 2014 Cell surface nationallicence Organic solvents nationallicence Saccharomyces cerevisiae nationallicence Stress resistance nationallicence Yeast surface display nationallicence Perpiñá C. Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, Dr. Moliner, 50, E-46100, Burjassot (València), Spain aut Vinaixa J. Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, Dr. Moliner, 50, E-46100, Burjassot (València), Spain aut Andreu C. Departament de Química Orgànica, Facultat de Farmàcia, Universitat de València, Vicent Andrés Estellés s/n, E-46100, Burjassot (València), Spain aut del Olmo M. Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, Dr. Moliner, 50, E-46100, Burjassot (València), Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 775-789 0175-7598 99:2<775 2015 99 253 https://doi.org/10.1007/s00253-014-6048-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6048-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Perpiñá C. Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, Dr. Moliner, 50, E-46100, Burjassot (València), Spain aut NATIONALLICENCE 700 1- Vinaixa J. Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, Dr. Moliner, 50, E-46100, Burjassot (València), Spain aut NATIONALLICENCE 700 1- Andreu C. Departament de Química Orgànica, Facultat de Farmàcia, Universitat de València, Vicent Andrés Estellés s/n, E-46100, Burjassot (València), Spain aut NATIONALLICENCE 700 1- del Olmo M. Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, Dr. Moliner, 50, E-46100, Burjassot (València), Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 775-789 0175-7598 99:2<775 2015 99 253