Engineering Clostridium acetobutylicum with a histidine kinase knockout for enhanced n -butanol tolerance and production
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 60550511X | ||
| 003 | CHVBK | ||
| 005 | 20210128100620.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150101xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-014-6249-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6249-7 | ||
| 245 | 0 | 0 | |a Engineering Clostridium acetobutylicum with a histidine kinase knockout for enhanced n -butanol tolerance and production |h [Elektronische Daten] |c [Mengmeng Xu, Jingbo Zhao, Le Yu, I-Ching Tang, Chuang Xue, Shang-Tian Yang] |
| 520 | 3 | |a Clostridium acetobutylicum JB200, a mutant strain of C. acetobutylicum ATCC 55025 obtained through strain evolution in a fibrous bed bioreactor, had high butanol tolerance and produced up to ~21g/L butanol from glucose in batch fermentation, an improvement of ~67% over the parental strain (~12.6g/L). Comparative genomic analysis revealed a single-base deletion in the cac3319 gene leading to C-terminal truncation in its encoding histidine kinase (HK) in JB200. To study the effects of cac3319 mutation on cell growth and fermentation, the cac3319 gene in ATCC 55025 was disrupted using the ClosTron group II intron-based gene inactivation system. Compared to ATCC 55025, the cac3319 HK knockout mutant, HKKO, produced 44.4% more butanol (18.2 ± 1.3 vs. 12.6 ± 0.2g/L) with a 90% higher productivity (0.38 ± 0.03 vs. 0.20 ± 0.02g/Lh) due to increased butanol tolerance, confirming, for the first time, that cac3319 plays an important role in regulating solvent production and tolerance in C. acetobutylicum. This work also provides a novel metabolic engineering strategy for generating high-butanol-tolerant and high-butanol-producing strains for industrial applications. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a ABE fermentation |2 nationallicence | |
| 690 | 7 | |a Butanol tolerance |2 nationallicence | |
| 690 | 7 | |a C. acetobutylicum |2 nationallicence | |
| 690 | 7 | |a Histidine kinase |2 nationallicence | |
| 690 | 7 | |a Gene knockout |2 nationallicence | |
| 690 | 7 | |a Metabolic engineering |2 nationallicence | |
| 700 | 1 | |a Xu |D Mengmeng |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave, 43210, Columbus, OH, USA |4 aut | |
| 700 | 1 | |a Zhao |D Jingbo |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave, 43210, Columbus, OH, USA |4 aut | |
| 700 | 1 | |a Yu |D Le |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave, 43210, Columbus, OH, USA |4 aut | |
| 700 | 1 | |a Tang |D I-Ching |u Bioprocessing Innovative Company, 4734 Bridle Path Ct, 43017, Dublin, OH, USA |4 aut | |
| 700 | 1 | |a Xue |D Chuang |u Department of Life Science and Biotechnology, Dalian University of Technology, 116024, Dalian, China |4 aut | |
| 700 | 1 | |a Yang |D Shang-Tian |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave, 43210, Columbus, OH, USA |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 1011-1022 |x 0175-7598 |q 99:2<1011 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6249-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6249-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xu |D Mengmeng |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave, 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhao |D Jingbo |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave, 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Le |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave, 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tang |D I-Ching |u Bioprocessing Innovative Company, 4734 Bridle Path Ct, 43017, Dublin, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xue |D Chuang |u Department of Life Science and Biotechnology, Dalian University of Technology, 116024, Dalian, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yang |D Shang-Tian |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave, 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 1011-1022 |x 0175-7598 |q 99:2<1011 |1 2015 |2 99 |o 253 | ||