caa a22 4500 605505144 CHVBK 20210128100620.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6115-7 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6115-7 A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina ( Diptera: Calliphoridae ) [Elektronische Daten] [Baneshwar Singh, Tawni Crippen, Longyu Zheng, Andrew Fields, Ziniu Yu, Qun Ma, Thomas Wood, Scot Dowd, Micah Flores, Jeffery Tomberlin, Aaron Tarone] Lucilia Robineau-Desvoidy (Diptera: Calliphoridae) is a blow fly genus of forensic, medical, veterinary, and agricultural importance. This genus is also famous because of its beneficial uses in maggot debridement therapy (MDT). Although the genus is of considerable economic importance, our knowledge about microbes associated with these flies and how these bacteria are horizontally and trans-generationally transmitted is limited. In this study, we characterized bacteria associated with different life stages of Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) and in the salivary gland of L. sericata by using 16S rDNA 454 pyrosequencing. Bacteria associated with the salivary gland of L. sericata were also characterized using light and transmission electron microscopy (TEM). Results from this study suggest that the majority of bacteria associated with these flies belong to phyla Proteobacteria, Firmicutes, and Bacteroidetes, and most bacteria are maintained intragenerationally, with a considerable degree of turnover from generation to generation. In both species, second-generation eggs exhibited the highest bacterial phylum diversity (20% genetic distance) than other life stages. The Lucilia sister species shared the majority of their classified genera. Of the shared bacterial genera, Providencia, Ignatzschineria, Lactobacillus, Lactococcus, Vagococcus, Morganella, and Myroides were present at relatively high abundances. Lactobacillus, Proteus, Diaphorobacter, and Morganella were the dominant bacterial genera associated with a survey of the salivary gland of L. sericata. TEM analysis showed a sparse distribution of both Gram-positive and Gram-negative bacteria in the salivary gland of L. sericata. There was more evidence for horizontal transmission of bacteria than there was for trans-generational inheritance. Several pathogenic genera were either amplified or reduced by the larval feeding on decomposing liver as a resource. Overall, this study provides information on bacterial communities associated with different life stages of Lucilia and their horizontal and trans-generational transmission, which may help in the development of better vector-borne disease management and MDT methods. Springer-Verlag Berlin Heidelberg, 2014 Microbial community nationallicence Blow flies nationallicence Maggot debridement therapy nationallicence Salivary gland nationallicence 454 sequencing nationallicence Singh Baneshwar Department of Entomology, Texas A&M University, College Station, TX, USA aut Crippen Tawni Southern Plains Agricultural Research Center, Agricultural Research Service, USDA, College Station, TX, USA aut Zheng Longyu Department of Entomology, Texas A&M University, College Station, TX, USA aut Fields Andrew Department of Entomology, Texas A&M University, College Station, TX, USA aut Yu Ziniu State Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticide, Huazhong Agricultural University, Wuhan, China aut Ma Qun Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin, China aut Wood Thomas Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA aut Dowd Scot MR DNA Molecular Research LP, Shallowater, TX, USA aut Flores Micah Department of Entomology, Texas A&M University, College Station, TX, USA aut Tomberlin Jeffery Department of Entomology, Texas A&M University, College Station, TX, USA aut Tarone Aaron Department of Entomology, Texas A&M University, College Station, TX, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 869-883 0175-7598 99:2<869 2015 99 253 https://doi.org/10.1007/s00253-014-6115-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6115-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Singh Baneshwar Department of Entomology, Texas A&M University, College Station, TX, USA aut NATIONALLICENCE 700 1- Crippen Tawni Southern Plains Agricultural Research Center, Agricultural Research Service, USDA, College Station, TX, USA aut NATIONALLICENCE 700 1- Zheng Longyu Department of Entomology, Texas A&M University, College Station, TX, USA aut NATIONALLICENCE 700 1- Fields Andrew Department of Entomology, Texas A&M University, College Station, TX, USA aut NATIONALLICENCE 700 1- Yu Ziniu State Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticide, Huazhong Agricultural University, Wuhan, China aut NATIONALLICENCE 700 1- Ma Qun Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin, China aut NATIONALLICENCE 700 1- Wood Thomas Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA aut NATIONALLICENCE 700 1- Dowd Scot MR DNA Molecular Research LP, Shallowater, TX, USA aut NATIONALLICENCE 700 1- Flores Micah Department of Entomology, Texas A&M University, College Station, TX, USA aut NATIONALLICENCE 700 1- Tomberlin Jeffery Department of Entomology, Texas A&M University, College Station, TX, USA aut NATIONALLICENCE 700 1- Tarone Aaron Department of Entomology, Texas A&M University, College Station, TX, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 869-883 0175-7598 99:2<869 2015 99 253