caa a22 4500 605505152 CHVBK 20210128100620.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6131-7 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6131-7 Inverse metabolic engineering of Bacillus subtilis for xylose utilization based on adaptive evolution and whole-genome sequencing [Elektronische Daten] [Bo Zhang, Ning Li, Zhiwen Wang, Ya-Jie Tang, Tao Chen, Xueming Zhao] Efficient utilization of xylose by bacteria is essential for production of fuels and chemicals from lignocellulosic biomass. In this study, Bacillus subtilis 168 was subjected to laboratory adaptive evolution, and a mutant E72, which could grow on xylose with a maximum specific growth rate of 0.445h−1, was obtained. By whole-genome sequencing, 16 mutations were identified in strain E72. Through further analysis, three of them, which were in the coding regions of genes araR, sinR, and comP, were identified as the beneficial mutations. The reconstructed strain 168ARSRCP harboring these three mutations exhibited similar growth capacity on xylose to the evolved strain E72, and the average xylose consumption rate of this strain is 0.530g/l/h, much higher than that of E72 (0.392g/l/h). Furthermore, genes acoA and bdhA were deleted and the final strain could utilize xylose to produce acetoin at 71% of the maximum theoretical yield. These results suggested that this strain could be used as a potential platform for production of fuels and chemicals from lignocellulosic biomass. Springer-Verlag Berlin Heidelberg, 2014 Bacillus subtilis nationallicence Xylose nationallicence Adaptive evolution nationallicence Whole-genome sequencing nationallicence Acetoin nationallicence Inverse metabolic engineering nationallicence Zhang Bo Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 300072, Tianjin, China aut Li Ning Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 300072, Tianjin, China aut Wang Zhiwen Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 300072, Tianjin, China aut Tang Ya-Jie Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, 430068, Wuhan, China aut Chen Tao Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 300072, Tianjin, China aut Zhao Xueming Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 300072, Tianjin, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 885-896 0175-7598 99:2<885 2015 99 253 https://doi.org/10.1007/s00253-014-6131-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6131-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Bo Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 300072, Tianjin, China aut NATIONALLICENCE 700 1- Li Ning Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 300072, Tianjin, China aut NATIONALLICENCE 700 1- Wang Zhiwen Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 300072, Tianjin, China aut NATIONALLICENCE 700 1- Tang Ya-Jie Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, 430068, Wuhan, China aut NATIONALLICENCE 700 1- Chen Tao Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 300072, Tianjin, China aut NATIONALLICENCE 700 1- Zhao Xueming Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 300072, Tianjin, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 885-896 0175-7598 99:2<885 2015 99 253