Enzymatic synthesis of poly(catechin)-antibiotic conjugates: an antimicrobial approach for indwelling catheters
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6128-2 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6128-2 | ||
| 245 | 0 | 0 | |a Enzymatic synthesis of poly(catechin)-antibiotic conjugates: an antimicrobial approach for indwelling catheters |h [Elektronische Daten] |c [Idalina Gonçalves, Ana Abreu, Teresa Matamá, Artur Ribeiro, Andreia Gomes, Carla Silva, Artur Cavaco-Paulo] |
| 520 | 3 | |a Biofilm formation in urinary indwelling catheters is one of the most critical issues that patients face. Catheters were coated with poly(catechin)-antibiotic conjugates with enhanced antimicrobial properties. Catechin was conjugated with two antibiotics, namely trimethoprim (TMP) and sulfamethoxazole (SMZ) via activation with N,N′-disuccinimidyl carbonate (DSC) and subsequent coupling to molecules containing α-amine moieties. Silicone and polyurethane catheters were functionalized in situ through laccase oxidation of catechin-antibiotic conjugates. Four antimicrobial coatings were produced, namely with poly(catechin), poly(catechin)-TMP, poly(catechin)-SMZ and poly(catechin)-TMP-SMZ. The bacterial adhesion reduction was tested on the functionalized devices using gram-negative and gram-positive strains. The most significant reduction in adhesion was observed with poly(catechin)-TMP (gram-negative −85% and gram-positive −87%) and with poly(catechin)-TMP-SMZ (gram-negative −85% and gram-positive −91%). The cytotoxicity to mammalian cells was tested by indirect contact for 5days and revealed that all the tested coatings supported more than 90% of viable cells. A promising approach for the increase of the indwelling catheter lifespan was developed aiming to reduce catheter-associated chronic infections. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Laccase |2 nationallicence | |
| 690 | 7 | |a Catechin |2 nationallicence | |
| 690 | 7 | |a Trimethoprim |2 nationallicence | |
| 690 | 7 | |a Sulfamethoxazole |2 nationallicence | |
| 690 | 7 | |a Antimicrobial |2 nationallicence | |
| 690 | 7 | |a Catheters |2 nationallicence | |
| 700 | 1 | |a Gonçalves |D Idalina |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | |
| 700 | 1 | |a Abreu |D Ana |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | |
| 700 | 1 | |a Matamá |D Teresa |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | |
| 700 | 1 | |a Ribeiro |D Artur |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | |
| 700 | 1 | |a Gomes |D Andreia |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | |
| 700 | 1 | |a Silva |D Carla |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | |
| 700 | 1 | |a Cavaco-Paulo |D Artur |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 637-651 |x 0175-7598 |q 99:2<637 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6128-2 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6128-2 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gonçalves |D Idalina |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Abreu |D Ana |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Matamá |D Teresa |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ribeiro |D Artur |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gomes |D Andreia |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Silva |D Carla |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cavaco-Paulo |D Artur |u CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 637-651 |x 0175-7598 |q 99:2<637 |1 2015 |2 99 |o 253 | ||