caa a22 4500 605505160 CHVBK 20210128100620.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6128-2 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6128-2 Enzymatic synthesis of poly(catechin)-antibiotic conjugates: an antimicrobial approach for indwelling catheters [Elektronische Daten] [Idalina Gonçalves, Ana Abreu, Teresa Matamá, Artur Ribeiro, Andreia Gomes, Carla Silva, Artur Cavaco-Paulo] Biofilm formation in urinary indwelling catheters is one of the most critical issues that patients face. Catheters were coated with poly(catechin)-antibiotic conjugates with enhanced antimicrobial properties. Catechin was conjugated with two antibiotics, namely trimethoprim (TMP) and sulfamethoxazole (SMZ) via activation with N,N′-disuccinimidyl carbonate (DSC) and subsequent coupling to molecules containing α-amine moieties. Silicone and polyurethane catheters were functionalized in situ through laccase oxidation of catechin-antibiotic conjugates. Four antimicrobial coatings were produced, namely with poly(catechin), poly(catechin)-TMP, poly(catechin)-SMZ and poly(catechin)-TMP-SMZ. The bacterial adhesion reduction was tested on the functionalized devices using gram-negative and gram-positive strains. The most significant reduction in adhesion was observed with poly(catechin)-TMP (gram-negative −85% and gram-positive −87%) and with poly(catechin)-TMP-SMZ (gram-negative −85% and gram-positive −91%). The cytotoxicity to mammalian cells was tested by indirect contact for 5days and revealed that all the tested coatings supported more than 90% of viable cells. A promising approach for the increase of the indwelling catheter lifespan was developed aiming to reduce catheter-associated chronic infections. Springer-Verlag Berlin Heidelberg, 2014 Laccase nationallicence Catechin nationallicence Trimethoprim nationallicence Sulfamethoxazole nationallicence Antimicrobial nationallicence Catheters nationallicence Gonçalves Idalina CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut Abreu Ana CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut Matamá Teresa CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut Ribeiro Artur CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut Gomes Andreia CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut Silva Carla CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut Cavaco-Paulo Artur CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 637-651 0175-7598 99:2<637 2015 99 253 https://doi.org/10.1007/s00253-014-6128-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6128-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gonçalves Idalina CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Abreu Ana CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Matamá Teresa CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Ribeiro Artur CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Gomes Andreia CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Silva Carla CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut NATIONALLICENCE 700 1- Cavaco-Paulo Artur CEB—Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 637-651 0175-7598 99:2<637 2015 99 253