caa a22 4500 605505187 CHVBK 20210128100620.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6130-8 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6130-8 Putrescine production via the agmatine deiminase pathway increases the growth of Lactococcus lactis and causes the alkalinization of the culture medium [Elektronische Daten] [Beatriz del Rio, Daniel Linares, Victor Ladero, Begoña Redruello, Maria Fernández, Maria Martin, Miguel Alvarez] Lactococcus lactis is the most important starter culture organism used in the dairy industry. Although L. lactis species have been awarded Qualified Presumption of Safety status by the European Food Safety Authority, and Generally Regarded as Safe status by the US Food and Drug Administration, some strains can produce the biogenic amine putrescine. One such strain is L. lactis subsp. cremoris CECT 8666 (formerly L. lactis subsp. cremoris GE2-14), which was isolated from Genestoso cheese. This strain catabolizes agmatine to putrescine via the agmatine deiminase (AGDI) pathway, which involves the production of ATP and two ammonium ions. The present work shows that the availability of agmatine and its metabolization to putrescine allows for greater bacterial growth (in a biphasic pattern) and causes the alkalinization of the culture medium in a dose-dependent manner. The construction of a mutant lacking the AGDI cluster (L. lactis CECT 8666 Δagdi) confirmed the latter's direct role in putrescine production, growth, and medium alkalinization. Alkalinization did not affect the putrescine production pattern and was not essential for increased bacterial growth. Springer-Verlag Berlin Heidelberg, 2014 Lactococcus lactis nationallicence Biogenic amines nationallicence Putrescine nationallicence Agmatine deiminase nationallicence AGDI cluster nationallicence Bacterial growth nationallicence del Rio Beatriz Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut Linares Daniel Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut Ladero Victor Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut Redruello Begoña Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut Fernández Maria Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut Martin Maria Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut Alvarez Miguel Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 897-905 0175-7598 99:2<897 2015 99 253 https://doi.org/10.1007/s00253-014-6130-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6130-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- del Rio Beatriz Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut NATIONALLICENCE 700 1- Linares Daniel Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut NATIONALLICENCE 700 1- Ladero Victor Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut NATIONALLICENCE 700 1- Redruello Begoña Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut NATIONALLICENCE 700 1- Fernández Maria Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut NATIONALLICENCE 700 1- Martin Maria Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut NATIONALLICENCE 700 1- Alvarez Miguel Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300, Villaviciosa, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 897-905 0175-7598 99:2<897 2015 99 253