caa a22 4500 605505195 CHVBK 20210128100620.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6311-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6311-5 Genome-wide identification, expression analysis of GH3 family genes in Medicago truncatula under stress-related hormones and Sinorhizobium meliloti infection [Elektronische Daten] [Yanjun Yang, Runqing Yue, Tao Sun, Lei Zhang, Wei Chen, Houqing Zeng, Huizhong Wang, Chenjia Shen] Auxin plays a pivotal role in the regulation of plant growth and development by controlling the expression of auxin response genes rapidly. As one of the major auxin early response gene families, Gretchen Hagen 3 (GH3) genes are involved in auxin homeostasis by conjugating excess auxins to amino acids. However, how GH3 genes function in environmental stresses and rhizobial infection responses in Medicago truncatula are largely unknown. Here, based on the latest updated M. truncatula genome, a comprehensive identification and expression profiling analysis of MtGH3 genes were performed. Our data showed that most of MtGH3 genes were expressed in tissue-specific manner and were responsive to environmental stress-related hormones. To understand the possible roles of MtGH3 genes involved in symbiosis establishment between M. truncatula and symbiotic bacteria, quantitative real-time polymerase chain reaction (qRT-PCR) was used to test the expressions of MtGH3 genes during the early phase of Sinorhizobium meliloti infection. The expression levels of most MtGH3 genes were upregulated in shoots and downregulated in roots by S. meliloti infection. The differences in expression responses to S. meliloti infection between roots and shoots were in agreement with the results of free indoleacetic acid (IAA) content measurements. The identification and expression analysis of MtGH3 genes at the early phase of S. meliloti infection may help us to understand the role of GH3-mediated IAA homeostasis in the regulation of nodule formation in model legumes M. truncatula. Springer-Verlag Berlin Heidelberg, 2014 Auxin nationallicence Auxin signaling nationallicence GH3 gene family nationallicence Medicago truncatula nationallicence Sinorhizobium meliloti nationallicence Phytohormone nationallicence Yang Yanjun College of Life and Environmental Sciences, Hangzhou Normal University, 310036, Hangzhou, China aut Yue Runqing Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China aut Sun Tao College of Life and Environmental Sciences, Hangzhou Normal University, 310036, Hangzhou, China aut Zhang Lei Department of Plant Pathology, Washington State University, 99164-6430, Pullman, WA, USA aut Chen Wei College of Life Sciences, Zhejiang University, Hangzhou, 310058, China aut Zeng Houqing College of Life and Environmental Sciences, Hangzhou Normal University, 310036, Hangzhou, China aut Wang Huizhong College of Life and Environmental Sciences, Hangzhou Normal University, 310036, Hangzhou, China aut Shen Chenjia College of Life and Environmental Sciences, Hangzhou Normal University, 310036, Hangzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 841-854 0175-7598 99:2<841 2015 99 253 https://doi.org/10.1007/s00253-014-6311-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6311-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Yanjun College of Life and Environmental Sciences, Hangzhou Normal University, 310036, Hangzhou, China aut NATIONALLICENCE 700 1- Yue Runqing Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China aut NATIONALLICENCE 700 1- Sun Tao College of Life and Environmental Sciences, Hangzhou Normal University, 310036, Hangzhou, China aut NATIONALLICENCE 700 1- Zhang Lei Department of Plant Pathology, Washington State University, 99164-6430, Pullman, WA, USA aut NATIONALLICENCE 700 1- Chen Wei College of Life Sciences, Zhejiang University, Hangzhou, 310058, China aut NATIONALLICENCE 700 1- Zeng Houqing College of Life and Environmental Sciences, Hangzhou Normal University, 310036, Hangzhou, China aut NATIONALLICENCE 700 1- Wang Huizhong College of Life and Environmental Sciences, Hangzhou Normal University, 310036, Hangzhou, China aut NATIONALLICENCE 700 1- Shen Chenjia College of Life and Environmental Sciences, Hangzhou Normal University, 310036, Hangzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 841-854 0175-7598 99:2<841 2015 99 253