caa a22 4500 605505209 CHVBK 20210128100620.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6228-z doi (NATIONALLICENCE)springer-10.1007/s00253-014-6228-z Engineering propionibacteria as versatile cell factories for the production of industrially important chemicals: advances, challenges, and prospects [Elektronische Daten] [Ningzi Guan, Xin Zhuge, Jianghua Li, Hyun-dong Shin, Jing Wu, Zhongping Shi, Long Liu] Propionibacteria are actinobacteria consisting of two principal groups: cutaneous and dairy. Cutaneous propionibacteria are considered primary pathogens to humans, whereas dairy propionibacteria are widely used in the food and pharmaceutical industries. Increasing attention has been focused on improving the performance of dairy propionibacteria for the production of industrially important chemicals, and significant advances have been made through strain engineering and process optimization in the production of flavor compounds, nutraceuticals, and antimicrobial compounds. In addition, genome sequencing of several propionibacteria species has been completed, deepening understanding of the metabolic and physiological features of these organisms. However, the metabolic engineering of propionibacteria still faces several challenges owing to the lack of efficient genome manipulation tools and the existence of various types of strong restriction-modification systems. The emergence of systems and synthetic biology provides new opportunities to overcome these bottlenecks. In this review, we first introduce the major species of propionibacteria and their properties and provide an overview of their functions and applications. We then discuss advances in the genome sequencing and metabolic engineering of these bacteria. Finally, we discuss systems and synthetic biology approaches for engineering propionibacteria as efficient and robust cell factories for the production of industrially important chemicals. Springer-Verlag Berlin Heidelberg, 2014 Propionibacteria nationallicence Genome sequencing nationallicence Metabolic engineering nationallicence Synthetic biology nationallicence Systems biology nationallicence Cell factories nationallicence Guan Ningzi Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China aut Zhuge Xin Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China aut Li Jianghua Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China aut Shin Hyun-dong School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 30332, Atlanta, GA, USA aut Wu Jing State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China aut Shi Zhongping Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China aut Liu Long Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 585-600 0175-7598 99:2<585 2015 99 253 https://doi.org/10.1007/s00253-014-6228-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6228-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guan Ningzi Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Zhuge Xin Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Li Jianghua Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Shin Hyun-dong School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 30332, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Wu Jing State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Shi Zhongping Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Liu Long Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 585-600 0175-7598 99:2<585 2015 99 253