caa a22 4500 605505217 CHVBK 20210128100620.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6124-6 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6124-6 The role of three calcineurin subunits and a related transcription factor (Crz1) in conidiation, multistress tolerance and virulence in Beauveria bassiana [Elektronische Daten] [Fang Li, Zheng-Liang Wang, Long-Bin Zhang, Sheng-Hua Ying, Ming-Guang Feng] The eukaryotic calcineurin (CN) pathway comprising catalytic A (CnA) and regulatory B subunits (CnB) is crucial for many biological processes but functionally unexplored in entomopathogenic fungi. Here, we characterise three CN subunits (CnA1, CnA2 and CnB) and a downstream CN-responsive zinc finger transcription factor (Crz1) in Beauveria bassiana. CN-mediated phosphatase activity decreased by 16-38% in all deletion mutants compared with wild type. Growth and conidiation were most defective in ΔcnB, which showed a large proportion of abnormally branched germlings but were less defective in ΔcnA1 and ΔcnA2. Conidiation defects also occurred in Δcrz1, uniquely accompanied with slower germination. Compared with wild type, the four deletion mutants became, to varying degrees, more sensitive to Ca2+, Mn2+, Zn2+, Mg2+, two oxidants, three cell wall stressors, carbendazim, heat shock and ultraviolet (UV)-B irradiation. They were also less virulent to Spodoptera litura larvae. Only ΔcnB and Δcrz1 were less tolerant to high osmolarity. The altered phenotypes of the deletion mutants were associated with lower intracellular mannitol and trehalose levels, reduced overall activity of superoxide dismutases and catalases, altered cell wall composition and down-regulation of numerous phenotype-influencing genes. Additionally, the transcription of six cascaded genes in two stress-responsive mitogen-activated protein kinase (MAPK) pathways and the phosphorylation of hallmarking Hog1 and Slt2 were largely down-regulated in all the deletion mutants under osmotic and cell wall stresses, respectively. All the changes were restored by gene complementation. Taken together, three calcineurin subunits and Crz1 play vital, but variable, roles in B. bassiana responses to environmental stresses during development and host signals during infection. Springer-Verlag Berlin Heidelberg, 2014 Entomopathogenic fungi nationallicence Calcineurin pathway nationallicence Functional diversity nationallicence Asexual development nationallicence Stress response nationallicence Virulence nationallicence Li Fang Institute of Microbiology, College of Life Sciences, Zhejiang University, 310058, Hangzhou, Zhejiang, China aut Wang Zheng-Liang Institute of Microbiology, College of Life Sciences, Zhejiang University, 310058, Hangzhou, Zhejiang, China aut Zhang Long-Bin Institute of Microbiology, College of Life Sciences, Zhejiang University, 310058, Hangzhou, Zhejiang, China aut Ying Sheng-Hua Institute of Microbiology, College of Life Sciences, Zhejiang University, 310058, Hangzhou, Zhejiang, China aut Feng Ming-Guang Institute of Microbiology, College of Life Sciences, Zhejiang University, 310058, Hangzhou, Zhejiang, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 827-840 0175-7598 99:2<827 2015 99 253 https://doi.org/10.1007/s00253-014-6124-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6124-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Fang Institute of Microbiology, College of Life Sciences, Zhejiang University, 310058, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Wang Zheng-Liang Institute of Microbiology, College of Life Sciences, Zhejiang University, 310058, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Zhang Long-Bin Institute of Microbiology, College of Life Sciences, Zhejiang University, 310058, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Ying Sheng-Hua Institute of Microbiology, College of Life Sciences, Zhejiang University, 310058, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Feng Ming-Guang Institute of Microbiology, College of Life Sciences, Zhejiang University, 310058, Hangzhou, Zhejiang, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 827-840 0175-7598 99:2<827 2015 99 253