caa a22 4500 605505225 CHVBK 20210128100620.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6231-4 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6231-4 Introducing capnophilic lactic fermentation in a combined dark-photo fermentation process: a route to unparalleled H2 yields [Elektronische Daten] [L. Dipasquale, A. Adessi, G. d'Ippolito, F. Rossi, A. Fontana, R. De Philippis] Two-stage process based on photofermentation of dark fermentation effluents is widely recognized as the most effective method for biological production of hydrogen from organic substrates. Recently, it was described an alternative mechanism, named capnophilic lactic fermentation, for sugar fermentation by the hyperthermophilic bacterium Thermotoga neapolitana in CO2-rich atmosphere. Here, we report the first application of this novel process to two-stage biological production of hydrogen. The microbial system based on T. neapolitana DSM 4359T and Rhodopseudomonas palustris 42OL gave 9.4mol of hydrogen per mole of glucose consumed during the anaerobic process, which is the best production yield so far reported for conventional two-stage batch cultivations. The improvement of hydrogen yield correlates with the increase in lactic production during capnophilic lactic fermentation and takes also advantage of the introduction of original conditions for culturing both microorganisms in minimal media based on diluted sea water. The use of CO2 during the first step of the combined process establishes a novel strategy for biohydrogen technology. Moreover, this study opens the way to cost reduction and use of salt-rich waste as feedstock. Springer-Verlag Berlin Heidelberg, 2014 Biological hydrogen production nationallicence Capnophilic lactic fermentation nationallicence Photofermentation nationallicence Thermotoga neapolitana nationallicence Rhodopseudomonas palustris nationallicence Dipasquale L. Institute of Biomolecular Chemistry (ICB), CNR, Via Campi Flegrei 34, 80078, Pozzuoli, Napoli, Italy aut Adessi A. Department of Agrifood Production and Environmental Sciences, University of Florence, Piazzale delle Cascine 24, 50144, Florence, Italy aut d'Ippolito G. Institute of Biomolecular Chemistry (ICB), CNR, Via Campi Flegrei 34, 80078, Pozzuoli, Napoli, Italy aut Rossi F. Department of Agrifood Production and Environmental Sciences, University of Florence, Piazzale delle Cascine 24, 50144, Florence, Italy aut Fontana A. Institute of Biomolecular Chemistry (ICB), CNR, Via Campi Flegrei 34, 80078, Pozzuoli, Napoli, Italy aut De Philippis R. Department of Agrifood Production and Environmental Sciences, University of Florence, Piazzale delle Cascine 24, 50144, Florence, Italy aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 1001-1010 0175-7598 99:2<1001 2015 99 253 https://doi.org/10.1007/s00253-014-6231-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6231-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dipasquale L. Institute of Biomolecular Chemistry (ICB), CNR, Via Campi Flegrei 34, 80078, Pozzuoli, Napoli, Italy aut NATIONALLICENCE 700 1- Adessi A. Department of Agrifood Production and Environmental Sciences, University of Florence, Piazzale delle Cascine 24, 50144, Florence, Italy aut NATIONALLICENCE 700 1- d'Ippolito G. Institute of Biomolecular Chemistry (ICB), CNR, Via Campi Flegrei 34, 80078, Pozzuoli, Napoli, Italy aut NATIONALLICENCE 700 1- Rossi F. Department of Agrifood Production and Environmental Sciences, University of Florence, Piazzale delle Cascine 24, 50144, Florence, Italy aut NATIONALLICENCE 700 1- Fontana A. Institute of Biomolecular Chemistry (ICB), CNR, Via Campi Flegrei 34, 80078, Pozzuoli, Napoli, Italy aut NATIONALLICENCE 700 1- De Philippis R. Department of Agrifood Production and Environmental Sciences, University of Florence, Piazzale delle Cascine 24, 50144, Florence, Italy aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 1001-1010 0175-7598 99:2<1001 2015 99 253