caa a22 4500 605505233 CHVBK 20210128100620.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6253-y doi (NATIONALLICENCE)springer-10.1007/s00253-014-6253-y Hydrogels for 3D mammalian cell culture: a starting guide for laboratory practice [Elektronische Daten] [Ferdinand Ruedinger, Antonina Lavrentieva, Cornelia Blume, Iliyana Pepelanova, Thomas Scheper] Hydrogels have become one of the most popular platforms for three-dimensional (3D) cultivation of mammalian cells. The enormous versatility of hydrogel materials makes it possible to design scaffolds with predefined mechanical properties, as well as with desired biofunctionality. 3D hydrogel constructs have been used for a variety of applications, including tissue engineering of microorgan systems, drug delivery, cytotoxicity testing, and drug screening. Moreover, 3D culture is applied for investigating cellular physiology, stem cell differentiation, and tumor models and for studying interaction mechanisms between the extracellular matrix and cells. In this paper, we review current examples of performance-based hydrogel design for 3D cell culture applications. A major emphasis is placed on a description of how standard analytical protocols and imaging techniques are being adapted to analysis of 3D cell culture in hydrogel systems. Springer-Verlag Berlin Heidelberg, 2014 Hydrogel nationallicence 3D cell culture nationallicence Mammalian cell culture nationallicence 3D analytics nationallicence Material design nationallicence Ruedinger Ferdinand Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstraße 5, 30167, Hanover, Germany aut Lavrentieva Antonina Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstraße 5, 30167, Hanover, Germany aut Blume Cornelia Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstraße 5, 30167, Hanover, Germany aut Pepelanova Iliyana Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstraße 5, 30167, Hanover, Germany aut Scheper Thomas Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstraße 5, 30167, Hanover, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 623-636 0175-7598 99:2<623 2015 99 253 https://doi.org/10.1007/s00253-014-6253-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6253-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ruedinger Ferdinand Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstraße 5, 30167, Hanover, Germany aut NATIONALLICENCE 700 1- Lavrentieva Antonina Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstraße 5, 30167, Hanover, Germany aut NATIONALLICENCE 700 1- Blume Cornelia Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstraße 5, 30167, Hanover, Germany aut NATIONALLICENCE 700 1- Pepelanova Iliyana Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstraße 5, 30167, Hanover, Germany aut NATIONALLICENCE 700 1- Scheper Thomas Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstraße 5, 30167, Hanover, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 623-636 0175-7598 99:2<623 2015 99 253