Bead-based suspension array for simultaneous differential detection of five major swine viruses
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605505241 | ||
| 003 | CHVBK | ||
| 005 | 20210128100621.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150101xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-014-6337-8 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6337-8 | ||
| 245 | 0 | 0 | |a Bead-based suspension array for simultaneous differential detection of five major swine viruses |h [Elektronische Daten] |c [Ru Chen, Xiao-Lu Yu, Xiao-Bo Gao, Cun-Yi Xue, Chang-Xu Song, Yan Li, Yong-Chang Cao] |
| 520 | 3 | |a A novel multiplex detection array based on Luminex xMAP technology was developed and validated for simultaneous detection of five major viruses causing swine reproductive diseases. By combining one-step asymmetric multiplex reverse transcription polymerase chain reaction (RT-PCR) with xMAP bead-based hybridization and flow cytometry analysis, the resulting multiplex assay was capable of detecting single and mixed infections of PRRSV, PCV-2, PRV, CSFV, and PPV in a single reaction. The assay accurately detected and differentiated 23 viral strains used in this study. The low detection limit was determined as 2.2-22copies/μL (corresponding to 0.5-6.8fg/μL DNA template) on plasmid constructs containing viral fragments. The intra-assay and inter-assay variances (CV%) were low that ranged from 2.5 to 5.4% and 4.1 to 7.6%, respectively. The assay was applied to test field samples and detected single and mixed viral infections. The detection rate was higher than that of uniplex conventional PCR and RT-PCR methods. The detection of PRRSV by the bead-based multiplex assay was comparable with a commercially available real time RT-PCR kit. The test procedure on purified DNA or RNA samples could be completed within 2h. In conclusion, the bead-based suspension array presented here proved to be a high-throughput practical tool that provided highly specific and sensitive identification of single and multiple infections of five major viruses in pigs and boar semen. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a xMAP technology |2 nationallicence | |
| 690 | 7 | |a Bead-based suspension array |2 nationallicence | |
| 690 | 7 | |a Swine virus |2 nationallicence | |
| 690 | 7 | |a Multiplex detection |2 nationallicence | |
| 700 | 1 | |a Chen |D Ru |u Animal Inspection and Quarantine Laboratory, Technical Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, No.66 Huacheng Dadao Ave., Zhujiangxincheng, 510623, Guangzhou, China |4 aut | |
| 700 | 1 | |a Yu |D Xiao-Lu |u School of Life Sciences, Sun Yat-Sen University, Guangzhou, China |4 aut | |
| 700 | 1 | |a Gao |D Xiao-Bo |u Department of Genetics, National Research Institute for Family Planning, 100081, Beijing, China |4 aut | |
| 700 | 1 | |a Xue |D Cun-Yi |u School of Life Sciences, Sun Yat-Sen University, Guangzhou, China |4 aut | |
| 700 | 1 | |a Song |D Chang-Xu |u Veterinary Medicine Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China |4 aut | |
| 700 | 1 | |a Li |D Yan |u Veterinary Medicine Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China |4 aut | |
| 700 | 1 | |a Cao |D Yong-Chang |u School of Life Sciences, Sun Yat-Sen University, Guangzhou, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 919-928 |x 0175-7598 |q 99:2<919 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6337-8 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6337-8 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chen |D Ru |u Animal Inspection and Quarantine Laboratory, Technical Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, No.66 Huacheng Dadao Ave., Zhujiangxincheng, 510623, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Xiao-Lu |u School of Life Sciences, Sun Yat-Sen University, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gao |D Xiao-Bo |u Department of Genetics, National Research Institute for Family Planning, 100081, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xue |D Cun-Yi |u School of Life Sciences, Sun Yat-Sen University, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Song |D Chang-Xu |u Veterinary Medicine Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Yan |u Veterinary Medicine Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cao |D Yong-Chang |u School of Life Sciences, Sun Yat-Sen University, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 919-928 |x 0175-7598 |q 99:2<919 |1 2015 |2 99 |o 253 | ||