caa a22 4500 60550525X CHVBK 20210128100621.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6196-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6196-3 Niessen Ludwig Technische Universität München, Lehrstuhl für Technische Mikrobiologie, Gregor-Mendel-Straße 4, 85354, Freising, Germany aut Current state and future perspectives of loop-mediated isothermal amplification (LAMP)-based diagnosis of filamentous fungi and yeasts [Elektronische Daten] [Ludwig Niessen] Loop-mediated isothermal amplification is a rather novel method of enzymatic deoxyribonucleic acid amplification which can be applied for the diagnosis of viruses, bacteria, and fungi. Although firmly established in viral and bacterial diagnosis, the technology has only recently been applied to a noteworthy number of species in the filamentous fungi and yeasts. The current review gives an overview of the literature so far published on the topic by discussing the different groups of fungal organisms to which the method has been applied. Moreover, the method is described in detail as well as the different possibilities available for signal detection and quantification and sample preparation. Future perspective of loop-mediated isothermal amplification-based assays is discussed in the light of applicability for fungal diagnostics. Springer-Verlag Berlin Heidelberg, 2014 Fungi nationallicence Yeast nationallicence Detection nationallicence Identification nationallicence Loop-mediated isothermal amplification nationallicence Primer design nationallicence Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 553-574 0175-7598 99:2<553 2015 99 253 https://doi.org/10.1007/s00253-014-6196-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6196-3 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Niessen Ludwig Technische Universität München, Lehrstuhl für Technische Mikrobiologie, Gregor-Mendel-Straße 4, 85354, Freising, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 553-574 0175-7598 99:2<553 2015 99 253