Effect of inoculum sources on the enrichment of nitrite-dependent anaerobic methane-oxidizing bacteria

Verfasser / Beitragende:
[Zhanfei He, Chen Cai, Lidong Shen, Liping Lou, Ping Zheng, Xinhua Xu, Baolan Hu]
Ort, Verlag, Jahr:
2015
Enthalten in:
Applied Microbiology and Biotechnology, 99/2(2015-01-01), 939-946
Format:
Artikel (online)
ID: 605505306
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024 7 0 |a 10.1007/s00253-014-6033-8  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s00253-014-6033-8 
245 0 0 |a Effect of inoculum sources on the enrichment of nitrite-dependent anaerobic methane-oxidizing bacteria  |h [Elektronische Daten]  |c [Zhanfei He, Chen Cai, Lidong Shen, Liping Lou, Ping Zheng, Xinhua Xu, Baolan Hu] 
520 3 |a Nitrite-dependent anaerobic methane oxidation (n-damo) is a newly discovered biological process that couples anaerobic oxidation of methane (AOM) to nitrite reduction. In this study, three different inocula, methanogenic sludge, paddy soil, and freshwater sediment were used to enrich n-damo bacteria in three sequencing batch reactors (SBRs), and three n-damo enrichment cultures, C1, C2 and C3, were obtained, respectively. After 500days of incubation, Methylomirabilis oxyfera-like bacteria and n-damo activities were observed in cultures C1, C2, and C3, and the specific activities were 0.8 ± 0.1, 1.4 ± 0.1, and 1.0 ± 0.1μmol CH4 h−1g−1 VSS, respectively. The copy numbers of 16S rRNA genes from cultures C1, C2, and C3 were 5.0 ± 0.4 × 108, 6.1 ± 0.1 × 109, and 1.0 ± 0.2 × 109 copiesg−1 dry weight, respectively. The results indicated that paddy soil is an excellent inoculum for n-damo bacterial enrichment. This work expanded the alternative source of n-damo inoculum and benefited the further research of n-damo process. 
540 |a Springer-Verlag Berlin Heidelberg, 2014 
690 7 |a n-damo  |2 nationallicence 
690 7 |a Inoculum  |2 nationallicence 
690 7 |a Paddy soil  |2 nationallicence 
690 7 |a Methanogenic sludge  |2 nationallicence 
690 7 |a Freshwater sediment  |2 nationallicence 
700 1 |a He  |D Zhanfei  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
700 1 |a Cai  |D Chen  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
700 1 |a Shen  |D Lidong  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
700 1 |a Lou  |D Liping  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
700 1 |a Zheng  |D Ping  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
700 1 |a Xu  |D Xinhua  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
700 1 |a Hu  |D Baolan  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
773 0 |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/2(2015-01-01), 939-946  |x 0175-7598  |q 99:2<939  |1 2015  |2 99  |o 253 
856 4 0 |u https://doi.org/10.1007/s00253-014-6033-8  |q text/html  |z Onlinezugriff via DOI 
898 |a BK010053  |b XK010053  |c XK010000 
900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a research-article  |2 jats 
949 |B NATIONALLICENCE  |F NATIONALLICENCE  |b NL-springer 
950 |B NATIONALLICENCE  |P 856  |E 40  |u https://doi.org/10.1007/s00253-014-6033-8  |q text/html  |z Onlinezugriff via DOI 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a He  |D Zhanfei  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Cai  |D Chen  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Shen  |D Lidong  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Lou  |D Liping  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Zheng  |D Ping  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Xu  |D Xinhua  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Hu  |D Baolan  |u Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/2(2015-01-01), 939-946  |x 0175-7598  |q 99:2<939  |1 2015  |2 99  |o 253