caa a22 4500 605505306 CHVBK 20210128100621.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6033-8 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6033-8 Effect of inoculum sources on the enrichment of nitrite-dependent anaerobic methane-oxidizing bacteria [Elektronische Daten] [Zhanfei He, Chen Cai, Lidong Shen, Liping Lou, Ping Zheng, Xinhua Xu, Baolan Hu] Nitrite-dependent anaerobic methane oxidation (n-damo) is a newly discovered biological process that couples anaerobic oxidation of methane (AOM) to nitrite reduction. In this study, three different inocula, methanogenic sludge, paddy soil, and freshwater sediment were used to enrich n-damo bacteria in three sequencing batch reactors (SBRs), and three n-damo enrichment cultures, C1, C2 and C3, were obtained, respectively. After 500days of incubation, Methylomirabilis oxyfera-like bacteria and n-damo activities were observed in cultures C1, C2, and C3, and the specific activities were 0.8 ± 0.1, 1.4 ± 0.1, and 1.0 ± 0.1μmol CH4 h−1g−1 VSS, respectively. The copy numbers of 16S rRNA genes from cultures C1, C2, and C3 were 5.0 ± 0.4 × 108, 6.1 ± 0.1 × 109, and 1.0 ± 0.2 × 109 copiesg−1 dry weight, respectively. The results indicated that paddy soil is an excellent inoculum for n-damo bacterial enrichment. This work expanded the alternative source of n-damo inoculum and benefited the further research of n-damo process. Springer-Verlag Berlin Heidelberg, 2014 n-damo nationallicence Inoculum nationallicence Paddy soil nationallicence Methanogenic sludge nationallicence Freshwater sediment nationallicence He Zhanfei Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Cai Chen Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Shen Lidong Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Lou Liping Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Zheng Ping Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Xu Xinhua Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Hu Baolan Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 939-946 0175-7598 99:2<939 2015 99 253 https://doi.org/10.1007/s00253-014-6033-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6033-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He Zhanfei Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Cai Chen Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Shen Lidong Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Lou Liping Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Zheng Ping Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Xu Xinhua Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Hu Baolan Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 939-946 0175-7598 99:2<939 2015 99 253