caa a22 4500 605505314 CHVBK 20210128100621.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6240-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6240-3 Methylobacterium extorquens [Elektronische Daten] methylotrophy and biotechnological applications [Andrea Ochsner, Frank Sonntag, Markus Buchhaupt, Jens Schrader, Julia Vorholt] Methylotrophy is the ability to use reduced one-carbon compounds, such as methanol, as a single source of carbon and energy. Methanol is, due to its availability and potential for production from renewable resources, a valuable feedstock for biotechnology. Nature offers a variety of methylotrophic microorganisms that differ in their metabolism and represent resources for engineering of value-added products from methanol. The most extensively studied methylotroph is the Alphaproteobacterium Methylobacterium extorquens. Over the past five decades, the metabolism of M. extorquens has been investigated physiologically, biochemically, and more recently, using complementary omics technologies such as transcriptomics, proteomics, metabolomics, and fluxomics. These approaches, together with a genome-scale metabolic model, facilitate system-wide studies and the development of rational strategies for the successful generation of desired products from methanol. This review summarizes the knowledge of methylotrophy in M. extorquens, as well as the available tools and biotechnological applications. Springer-Verlag Berlin Heidelberg, 2014 Methylobacterium extorquens nationallicence Methanol nationallicence Methylotrophy nationallicence Industrial biotechnology nationallicence Ochsner Andrea Institute of Microbiology, ETH Zurich, Vladimir-Prelog-Weg 4, 8093, Zurich, Switzerland aut Sonntag Frank DECHEMA Forschungsinstitut, Theodor-Heuss-Allee 25, 60486, Frankfurt am Main, Germany aut Buchhaupt Markus DECHEMA Forschungsinstitut, Theodor-Heuss-Allee 25, 60486, Frankfurt am Main, Germany aut Schrader Jens DECHEMA Forschungsinstitut, Theodor-Heuss-Allee 25, 60486, Frankfurt am Main, Germany aut Vorholt Julia Institute of Microbiology, ETH Zurich, Vladimir-Prelog-Weg 4, 8093, Zurich, Switzerland aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 517-534 0175-7598 99:2<517 2015 99 253 https://doi.org/10.1007/s00253-014-6240-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6240-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ochsner Andrea Institute of Microbiology, ETH Zurich, Vladimir-Prelog-Weg 4, 8093, Zurich, Switzerland aut NATIONALLICENCE 700 1- Sonntag Frank DECHEMA Forschungsinstitut, Theodor-Heuss-Allee 25, 60486, Frankfurt am Main, Germany aut NATIONALLICENCE 700 1- Buchhaupt Markus DECHEMA Forschungsinstitut, Theodor-Heuss-Allee 25, 60486, Frankfurt am Main, Germany aut NATIONALLICENCE 700 1- Schrader Jens DECHEMA Forschungsinstitut, Theodor-Heuss-Allee 25, 60486, Frankfurt am Main, Germany aut NATIONALLICENCE 700 1- Vorholt Julia Institute of Microbiology, ETH Zurich, Vladimir-Prelog-Weg 4, 8093, Zurich, Switzerland aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 517-534 0175-7598 99:2<517 2015 99 253