caa a22 4500 605505322 CHVBK 20210128100621.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-5938-6 doi (NATIONALLICENCE)springer-10.1007/s00253-014-5938-6 Biogenic magnetic nanoparticles from Burkholderia sp. YN01 exhibiting intrinsic peroxidase-like activity and their applications [Elektronische Daten] [Yu Pan, Na Li, Jianshuai Mu, Runhong Zhou, Yan Xu, Daizong Cui, Yan Wang, Min Zhao] A novel bacterial strain containing biogenic magnetic nanoparticles (BMNPs) was isolated from the sediments of Songhua River in Harbin, China, and was identified as Burkholderia sp. YN01. Extracted BMNPs from YN01 were characterized as pure face-centered cubic Fe3O4 with an average size of 80nm through transmission electron microscope (TEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The hysteresis parameters of the BMNP samples such as Bc and Bcr and ratios Mrs/Ms were deduced as 35.6mT, 43.2mT, and 0.47, respectively, indicating that the BMNPs exhibit a ferromagnetic behavior. This is the first report concerning on biogenic Fe3O4 NPs produced in Burkholderia genus. Significantly, the BMNPs were proved to possess intrinsic peroxidase-like activity that could catalyze the oxidation of peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. Kinetic analysis indicates that the catalytic behavior is in accord with typical Michaelis-Menten kinetics and follows ping-pong mechanism. The catalytic constants (K cat) were 6.5 × 104s−1 and 0.78 × 104s−1 with H2O2 and TMB as substrate, respectively, which was higher than that of horseradish peroxidase (HRP). Electron spin resonance (ESR) spectroscopy experiments showed that the BMNPs could catalyze H2O2 to produce hydroxyl radicals. The origin of peroxidase-like activity is also associated with their ability to transfer electron between electrode and H2O2 according to an electrochemical study. As a novel peroxidase mimetic, the BMNPs were employed to offer a simple, sensitive, and selective colorimetric method for H2O2 and glucose determination, and the BMNPs could efficiently catalyze the degradation of phenol and Congo red dye. Springer-Verlag Berlin Heidelberg, 2014 Biogenic magnetic nanoparticles nationallicence Burkholderia nationallicence Peroxidase mimetic nationallicence Glucose determination nationallicence Degradation nationallicence Pan Yu College of Life Science, Northeast Forestry University, No. 26, Hexing Road, 150040, Harbin, China aut Li Na College of Life Science, Northeast Forestry University, No. 26, Hexing Road, 150040, Harbin, China aut Mu Jianshuai Academy of Fundamental and Interdisciplinary Sciences, Harbin Institute of Technology, 150001, Harbin, China aut Zhou Runhong College of Life Science, Northeast Forestry University, No. 26, Hexing Road, 150040, Harbin, China aut Xu Yan College of Life Science, Northeast Forestry University, No. 26, Hexing Road, 150040, Harbin, China aut Cui Daizong College of Life Science, Northeast Forestry University, No. 26, Hexing Road, 150040, Harbin, China aut Wang Yan Academy of Fundamental and Interdisciplinary Sciences, Harbin Institute of Technology, 150001, Harbin, China aut Zhao Min College of Life Science, Northeast Forestry University, No. 26, Hexing Road, 150040, Harbin, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 703-715 0175-7598 99:2<703 2015 99 253 https://doi.org/10.1007/s00253-014-5938-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-5938-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pan Yu College of Life Science, Northeast Forestry University, No. 26, Hexing Road, 150040, Harbin, China aut NATIONALLICENCE 700 1- Li Na College of Life Science, Northeast Forestry University, No. 26, Hexing Road, 150040, Harbin, China aut NATIONALLICENCE 700 1- Mu Jianshuai Academy of Fundamental and Interdisciplinary Sciences, Harbin Institute of Technology, 150001, Harbin, China aut NATIONALLICENCE 700 1- Zhou Runhong College of Life Science, Northeast Forestry University, No. 26, Hexing Road, 150040, Harbin, China aut NATIONALLICENCE 700 1- Xu Yan College of Life Science, Northeast Forestry University, No. 26, Hexing Road, 150040, Harbin, China aut NATIONALLICENCE 700 1- Cui Daizong College of Life Science, Northeast Forestry University, No. 26, Hexing Road, 150040, Harbin, China aut NATIONALLICENCE 700 1- Wang Yan Academy of Fundamental and Interdisciplinary Sciences, Harbin Institute of Technology, 150001, Harbin, China aut NATIONALLICENCE 700 1- Zhao Min College of Life Science, Northeast Forestry University, No. 26, Hexing Road, 150040, Harbin, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 703-715 0175-7598 99:2<703 2015 99 253