caa a22 4500 605505330 CHVBK 20210128100621.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-5977-z doi (NATIONALLICENCE)springer-10.1007/s00253-014-5977-z Simultaneous saccharification and fermentation of solid household waste following mild pretreatment using a mix of hydrolytic enzymes in combination with Saccharomyces cerevisiae [Elektronische Daten] [A. Nwobi, I. Cybulska, W. Tesfai, Y. Shatilla, J. Rodríguez, M. Thomsen] Ethanol production from low severity pretreated (85°C, 1h) solid household waste was studied using simultaneous saccharification and fermentation (SSF). The aim of the study was to examine typical composition of the organic fraction of municipal solid waste (OFMSW) and to develop a simple method for simultaneous liquefaction and biofuels production. A model waste was prepared based on the composition of the organic waste in Masdar City. Chemical analysis of the OFMSW showed that it contained 37% total solids with up to 57g glucan/100g total solid (TS). Hydrolysis of the wet OFMSW was carried out using a mix of hydrolytic enzymes: amylase, cellulase, protease, lipase, hemicellulase, and pectate lyase. The enzymatic hydrolysis using this enzyme mix was studied using different dilutions of the OFMSW at different enzyme loadings. This study has demonstrated that SSF of low severity pretreated OFMSW can be carried out using Saccharomyces cerevisiae without dilution (addition of water), and liquefaction of the undiluted OFMSW can be achieved in less than 24h of hydrolysis. Also, SSF of the pretreated waste can be carried out with very low enzyme loading (10% of the company recommended dosage)—0.1% cellulase, 0.1% amylase, 0.02% protease, 0.02% hemicellulase, 0.02% lipase, and 0.02% pectate lyase (w/w per TS) following mild heat pretreatment conditions of 85°C for 1h. Springer-Verlag Berlin Heidelberg, 2014 Enzyme nationallicence Ethanol nationallicence Hydrolysis nationallicence Fermentation nationallicence Waste nationallicence Nwobi A. Institute Center for Energy - iEnergy, Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, United Arab Emirates aut Cybulska I. Institute Center for Energy - iEnergy, Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, United Arab Emirates aut Tesfai W. Institute Center for Energy - iEnergy, Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, United Arab Emirates aut Shatilla Y. Institute Center for Energy - iEnergy, Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, United Arab Emirates aut Rodríguez J. Institute Centre for Water and Environment - iWater, Masdar Institute of Science and technology, P.O. Box 54224, Abu Dhabi, United Arab Emirates aut Thomsen M. Institute Center for Energy - iEnergy, Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, United Arab Emirates aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 929-938 0175-7598 99:2<929 2015 99 253 https://doi.org/10.1007/s00253-014-5977-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-5977-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nwobi A. Institute Center for Energy - iEnergy, Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, United Arab Emirates aut NATIONALLICENCE 700 1- Cybulska I. Institute Center for Energy - iEnergy, Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, United Arab Emirates aut NATIONALLICENCE 700 1- Tesfai W. Institute Center for Energy - iEnergy, Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, United Arab Emirates aut NATIONALLICENCE 700 1- Shatilla Y. Institute Center for Energy - iEnergy, Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, United Arab Emirates aut NATIONALLICENCE 700 1- Rodríguez J. Institute Centre for Water and Environment - iWater, Masdar Institute of Science and technology, P.O. Box 54224, Abu Dhabi, United Arab Emirates aut NATIONALLICENCE 700 1- Thomsen M. Institute Center for Energy - iEnergy, Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, United Arab Emirates aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 929-938 0175-7598 99:2<929 2015 99 253