An exceptionally cold-adapted alpha-amylase from a metagenomic library of a cold and alkaline environment
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| 024 | 7 | 0 | |a 10.1007/s00253-014-5931-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-5931-0 | ||
| 245 | 0 | 3 | |a An exceptionally cold-adapted alpha-amylase from a metagenomic library of a cold and alkaline environment |h [Elektronische Daten] |c [Jan Vester, Mikkel Glaring, Peter Stougaard] |
| 520 | 3 | |a A cold-active α-amylase, AmyI3C6, identified by a functional metagenomics approach was expressed in Escherichia coli and purified to homogeneity. Sequence analysis showed that the AmyI3C6 amylase was similar to α-amylases from the class Clostridia and revealed classical characteristics of cold-adapted enzymes, as did comparison of the kinetic parameters K m and k cat to a mesophilic α-amylase. AmyI3C6 was shown to be heat-labile. Temperature optimum was at 10-15°C, and more than 70% of the relative activity was retained at 1°C. The pH optimum of AmyI3C6 was at pH8-9, and the enzyme displayed activity in two commercial detergents tested, suggesting that the AmyI3C6 α-amylase may be useful as a detergent enzyme in environmentally friendly, low-temperature laundry processes. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a α-Amylase |2 nationallicence | |
| 690 | 7 | |a Cold-active |2 nationallicence | |
| 690 | 7 | |a Heat-labile |2 nationallicence | |
| 690 | 7 | |a Alkaline-active |2 nationallicence | |
| 690 | 7 | |a Detergents |2 nationallicence | |
| 690 | 7 | |a Clostridia |2 nationallicence | |
| 700 | 1 | |a Vester |D Jan |u Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Copenhagen, Denmark |4 aut | |
| 700 | 1 | |a Glaring |D Mikkel |u Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Copenhagen, Denmark |4 aut | |
| 700 | 1 | |a Stougaard |D Peter |u Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Copenhagen, Denmark |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 717-727 |x 0175-7598 |q 99:2<717 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-5931-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-5931-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Vester |D Jan |u Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Copenhagen, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Glaring |D Mikkel |u Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Copenhagen, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Stougaard |D Peter |u Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Copenhagen, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 717-727 |x 0175-7598 |q 99:2<717 |1 2015 |2 99 |o 253 | ||