caa a22 4500 605505357 CHVBK 20210128100621.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-5931-0 doi (NATIONALLICENCE)springer-10.1007/s00253-014-5931-0 An exceptionally cold-adapted alpha-amylase from a metagenomic library of a cold and alkaline environment [Elektronische Daten] [Jan Vester, Mikkel Glaring, Peter Stougaard] A cold-active α-amylase, AmyI3C6, identified by a functional metagenomics approach was expressed in Escherichia coli and purified to homogeneity. Sequence analysis showed that the AmyI3C6 amylase was similar to α-amylases from the class Clostridia and revealed classical characteristics of cold-adapted enzymes, as did comparison of the kinetic parameters K m and k cat to a mesophilic α-amylase. AmyI3C6 was shown to be heat-labile. Temperature optimum was at 10-15°C, and more than 70% of the relative activity was retained at 1°C. The pH optimum of AmyI3C6 was at pH8-9, and the enzyme displayed activity in two commercial detergents tested, suggesting that the AmyI3C6 α-amylase may be useful as a detergent enzyme in environmentally friendly, low-temperature laundry processes. Springer-Verlag Berlin Heidelberg, 2014 α-Amylase nationallicence Cold-active nationallicence Heat-labile nationallicence Alkaline-active nationallicence Detergents nationallicence Clostridia nationallicence Vester Jan Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Copenhagen, Denmark aut Glaring Mikkel Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Copenhagen, Denmark aut Stougaard Peter Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Copenhagen, Denmark aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 717-727 0175-7598 99:2<717 2015 99 253 https://doi.org/10.1007/s00253-014-5931-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-5931-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vester Jan Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Glaring Mikkel Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Stougaard Peter Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Copenhagen, Denmark aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 717-727 0175-7598 99:2<717 2015 99 253