Generation of an attenuated strain oral vaccine candidate using a novel double selection platform in Escherichia coli
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| 008 | 210128e20150101xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-014-6099-3 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6099-3 | ||
| 245 | 0 | 0 | |a Generation of an attenuated strain oral vaccine candidate using a novel double selection platform in Escherichia coli |h [Elektronische Daten] |c [Wenxin Liu, Chaowen Yuan, Jun Bao, Weikun Guan, Zhiteng Zhao, Xingyue Li, Jie Tang, Dandan Li, Dongfang Shi] |
| 520 | 3 | |a Live attenuated bacteria delivered orally are interesting tools for mucosal immunization. The objective of this study was to construct a novel counter-selection platform based on an attenuated wild-type Escherichia coli (E. coli) strain and to utilize it for the delivery of LTR192G-STaA13Q fusion protein as an oral vaccine. First, a counter-selectable marker, namely, PRPL-Kil, was inserted into an attenuated wild-type E. coli strain through the use of the red and G-DOC homologous recombination systems to construct the counter-selection platform, and PRPL-Kil was subsequently replaced by the LT192-STa13 fusion gene to construct the oral vaccine O142 (yaiT::LT192-STa13) (ER-A). Subsequently, BALB/c mice were orogastrically inoculated with ER-A. Our results showed that ER-A could induce the production of specific IgA and IgG against fimbriae (F41) and enterotoxins (LT and STa), with neutralizing activity in BALB/c mice. In addition, assays of cellular immune responses showed that the stimulation index (SI) values of immunized mice were significantly higher than those of control mice (P < 0.05), and revealed a marked shift toward Th2-mediated immunity. These findings suggest that ER-A is a suitable candidate for an oral vaccine strain to protect animals from enter toxigenic Escherichia coli (ETEC) infection. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Escherichia coli |2 nationallicence | |
| 690 | 7 | |a Mucosal vaccine |2 nationallicence | |
| 690 | 7 | |a Mice |2 nationallicence | |
| 690 | 7 | |a Enterotoxin |2 nationallicence | |
| 700 | 1 | |a Liu |D Wenxin |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | |
| 700 | 1 | |a Yuan |D Chaowen |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | |
| 700 | 1 | |a Bao |D Jun |u College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | |
| 700 | 1 | |a Guan |D Weikun |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | |
| 700 | 1 | |a Zhao |D Zhiteng |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | |
| 700 | 1 | |a Li |D Xingyue |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | |
| 700 | 1 | |a Tang |D Jie |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | |
| 700 | 1 | |a Li |D Dandan |u Heilongjiang Research Center for Stem Cell Engineering, 150028, Harbin, Heilongjiang, China |4 aut | |
| 700 | 1 | |a Shi |D Dongfang |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 855-867 |x 0175-7598 |q 99:2<855 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6099-3 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6099-3 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Wenxin |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yuan |D Chaowen |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bao |D Jun |u College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Guan |D Weikun |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhao |D Zhiteng |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Xingyue |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tang |D Jie |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Dandan |u Heilongjiang Research Center for Stem Cell Engineering, 150028, Harbin, Heilongjiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shi |D Dongfang |u Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 855-867 |x 0175-7598 |q 99:2<855 |1 2015 |2 99 |o 253 | ||