caa a22 4500 605505365 CHVBK 20210128100621.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6099-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6099-3 Generation of an attenuated strain oral vaccine candidate using a novel double selection platform in Escherichia coli [Elektronische Daten] [Wenxin Liu, Chaowen Yuan, Jun Bao, Weikun Guan, Zhiteng Zhao, Xingyue Li, Jie Tang, Dandan Li, Dongfang Shi] Live attenuated bacteria delivered orally are interesting tools for mucosal immunization. The objective of this study was to construct a novel counter-selection platform based on an attenuated wild-type Escherichia coli (E. coli) strain and to utilize it for the delivery of LTR192G-STaA13Q fusion protein as an oral vaccine. First, a counter-selectable marker, namely, PRPL-Kil, was inserted into an attenuated wild-type E. coli strain through the use of the red and G-DOC homologous recombination systems to construct the counter-selection platform, and PRPL-Kil was subsequently replaced by the LT192-STa13 fusion gene to construct the oral vaccine O142 (yaiT::LT192-STa13) (ER-A). Subsequently, BALB/c mice were orogastrically inoculated with ER-A. Our results showed that ER-A could induce the production of specific IgA and IgG against fimbriae (F41) and enterotoxins (LT and STa), with neutralizing activity in BALB/c mice. In addition, assays of cellular immune responses showed that the stimulation index (SI) values of immunized mice were significantly higher than those of control mice (P < 0.05), and revealed a marked shift toward Th2-mediated immunity. These findings suggest that ER-A is a suitable candidate for an oral vaccine strain to protect animals from enter toxigenic Escherichia coli (ETEC) infection. Springer-Verlag Berlin Heidelberg, 2014 Escherichia coli nationallicence Mucosal vaccine nationallicence Mice nationallicence Enterotoxin nationallicence Liu Wenxin Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut Yuan Chaowen Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut Bao Jun College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut Guan Weikun Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut Zhao Zhiteng Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut Li Xingyue Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut Tang Jie Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut Li Dandan Heilongjiang Research Center for Stem Cell Engineering, 150028, Harbin, Heilongjiang, China aut Shi Dongfang Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 855-867 0175-7598 99:2<855 2015 99 253 https://doi.org/10.1007/s00253-014-6099-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6099-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Wenxin Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut NATIONALLICENCE 700 1- Yuan Chaowen Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut NATIONALLICENCE 700 1- Bao Jun College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut NATIONALLICENCE 700 1- Guan Weikun Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut NATIONALLICENCE 700 1- Zhao Zhiteng Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut NATIONALLICENCE 700 1- Li Xingyue Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut NATIONALLICENCE 700 1- Tang Jie Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut NATIONALLICENCE 700 1- Li Dandan Heilongjiang Research Center for Stem Cell Engineering, 150028, Harbin, Heilongjiang, China aut NATIONALLICENCE 700 1- Shi Dongfang Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 855-867 0175-7598 99:2<855 2015 99 253