Mannose-modified chitosan microspheres enhance OprF-OprI-mediated protection of mice against Pseudomonas aeruginosa infection via induction of mucosal immunity
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605505381 | ||
| 003 | CHVBK | ||
| 005 | 20210128100621.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150101xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-014-6147-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6147-z | ||
| 245 | 0 | 0 | |a Mannose-modified chitosan microspheres enhance OprF-OprI-mediated protection of mice against Pseudomonas aeruginosa infection via induction of mucosal immunity |h [Elektronische Daten] |c [Ziyin Cui, Dong Han, Xiaoyu Sun, Mingliang Zhang, Xin Feng, Changjiang Sun, Jingmin Gu, Chunyu Tong, Liancheng Lei, Wenyu Han] |
| 520 | 3 | |a Pseudomonas aeruginosa is an opportunistic pathogen that localizes to and colonizes mucosal tissue. Thus, vaccines that elicit a strong mucosal response against P. aeruginosa should be superior to other vaccination strategies. In this study, to stimulate rapid and enhanced mucosal immune responses, mannose-modified chitosan microspheres loaded with the recombinant outer membrane protein OprF190-342-OprI21-83 (FI) (FI-MCS-MPs) of P. aeruginosa were developed as a potent subunit vaccine for mucosal delivery. FI-MCS-MPs were successfully obtained via the tripolyphosphate ionic crosslinking method. Confocal and immunohistochemical analyses indicated that FI-MCS-MPs exhibited the ability to bind the macrophage mannose receptor (MMR, CD206) in vitro and in vivo. After intranasal immunization of mice with FI-MCS-MPs, FI-specific humoral immune responses were detected, measured as local IgM antibody titers in lung tissue slurry; IgA antibody titers in nasal washes, bronchoalveolar lavage (BAL), and intestinal lavage; and systemic IgA and IgG antibody titers in serum. FI-MCS-MPs induced early and high mucosal and systemic humoral antibody responses comparable to those in the group vaccinated with unmodified mannose. High levels of IFN-γ and IL-4 in addition to T lymphocyte subsets induced a mixed Th1/Th2 response in mice immunized with FI-MCS-MPs, resulting in the establishment of cellular immunity. Additionally, when immunized mice were challenged with P. aeruginosa via the nasal cavity, FI-MCS-MPs demonstrated 75% protective efficacy. Together, these data indicate that mannose-modified chitosan microspheres are a promising subunit delivery system for vaccines against P. aeruginosa infection. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Mannose-modified chitosan microspheres |2 nationallicence | |
| 690 | 7 | |a Macrophage mannose receptor |2 nationallicence | |
| 690 | 7 | |a Pseudomonas aeruginosa outer membrane protein |2 nationallicence | |
| 690 | 7 | |a Intranasal immunization |2 nationallicence | |
| 700 | 1 | |a Cui |D Ziyin |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | |
| 700 | 1 | |a Han |D Dong |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | |
| 700 | 1 | |a Sun |D Xiaoyu |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | |
| 700 | 1 | |a Zhang |D Mingliang |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | |
| 700 | 1 | |a Feng |D Xin |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | |
| 700 | 1 | |a Sun |D Changjiang |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | |
| 700 | 1 | |a Gu |D Jingmin |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | |
| 700 | 1 | |a Tong |D Chunyu |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | |
| 700 | 1 | |a Lei |D Liancheng |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | |
| 700 | 1 | |a Han |D Wenyu |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 667-680 |x 0175-7598 |q 99:2<667 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6147-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6147-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cui |D Ziyin |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Han |D Dong |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sun |D Xiaoyu |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Mingliang |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Feng |D Xin |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sun |D Changjiang |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gu |D Jingmin |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tong |D Chunyu |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lei |D Liancheng |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Han |D Wenyu |u College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/2(2015-01-01), 667-680 |x 0175-7598 |q 99:2<667 |1 2015 |2 99 |o 253 | ||