caa a22 4500 605505381 CHVBK 20210128100621.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6147-z doi (NATIONALLICENCE)springer-10.1007/s00253-014-6147-z Mannose-modified chitosan microspheres enhance OprF-OprI-mediated protection of mice against Pseudomonas aeruginosa infection via induction of mucosal immunity [Elektronische Daten] [Ziyin Cui, Dong Han, Xiaoyu Sun, Mingliang Zhang, Xin Feng, Changjiang Sun, Jingmin Gu, Chunyu Tong, Liancheng Lei, Wenyu Han] Pseudomonas aeruginosa is an opportunistic pathogen that localizes to and colonizes mucosal tissue. Thus, vaccines that elicit a strong mucosal response against P. aeruginosa should be superior to other vaccination strategies. In this study, to stimulate rapid and enhanced mucosal immune responses, mannose-modified chitosan microspheres loaded with the recombinant outer membrane protein OprF190-342-OprI21-83 (FI) (FI-MCS-MPs) of P. aeruginosa were developed as a potent subunit vaccine for mucosal delivery. FI-MCS-MPs were successfully obtained via the tripolyphosphate ionic crosslinking method. Confocal and immunohistochemical analyses indicated that FI-MCS-MPs exhibited the ability to bind the macrophage mannose receptor (MMR, CD206) in vitro and in vivo. After intranasal immunization of mice with FI-MCS-MPs, FI-specific humoral immune responses were detected, measured as local IgM antibody titers in lung tissue slurry; IgA antibody titers in nasal washes, bronchoalveolar lavage (BAL), and intestinal lavage; and systemic IgA and IgG antibody titers in serum. FI-MCS-MPs induced early and high mucosal and systemic humoral antibody responses comparable to those in the group vaccinated with unmodified mannose. High levels of IFN-γ and IL-4 in addition to T lymphocyte subsets induced a mixed Th1/Th2 response in mice immunized with FI-MCS-MPs, resulting in the establishment of cellular immunity. Additionally, when immunized mice were challenged with P. aeruginosa via the nasal cavity, FI-MCS-MPs demonstrated 75% protective efficacy. Together, these data indicate that mannose-modified chitosan microspheres are a promising subunit delivery system for vaccines against P. aeruginosa infection. Springer-Verlag Berlin Heidelberg, 2014 Mannose-modified chitosan microspheres nationallicence Macrophage mannose receptor nationallicence Pseudomonas aeruginosa outer membrane protein nationallicence Intranasal immunization nationallicence Cui Ziyin College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut Han Dong College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut Sun Xiaoyu College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut Zhang Mingliang College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut Feng Xin College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut Sun Changjiang College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut Gu Jingmin College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut Tong Chunyu College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut Lei Liancheng College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut Han Wenyu College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 667-680 0175-7598 99:2<667 2015 99 253 https://doi.org/10.1007/s00253-014-6147-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6147-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cui Ziyin College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut NATIONALLICENCE 700 1- Han Dong College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut NATIONALLICENCE 700 1- Sun Xiaoyu College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut NATIONALLICENCE 700 1- Zhang Mingliang College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut NATIONALLICENCE 700 1- Feng Xin College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut NATIONALLICENCE 700 1- Sun Changjiang College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut NATIONALLICENCE 700 1- Gu Jingmin College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut NATIONALLICENCE 700 1- Tong Chunyu College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut NATIONALLICENCE 700 1- Lei Liancheng College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut NATIONALLICENCE 700 1- Han Wenyu College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, 130062, Changchun, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 667-680 0175-7598 99:2<667 2015 99 253