caa a22 4500 60550539X CHVBK 20210128100621.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6043-6 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6043-6 Algicidal activity of Bacillus sp. Lzh-5 and its algicidal compounds against Microcystis aeruginosa [Elektronische Daten] [Zhenghua Li, Mengxin Geng, Hong Yang] A freshwater algicidal bacterial strain, Lzh-5, isolated from Lake Taihu, with strong algicidal activity against Microcystis aeruginosa, was identified as Bacillus sp. based on its phenotypic characteristics and 16S ribosomal RNA (rRNA) gene sequence. The algicidal mode of Bacillus sp. Lzh-5 was indirect, attacking M. aeruginosa cells by releasing algicidal compounds. Two algicidal compounds (S-5A and S-5B) produced by Bacillus sp. Lzh-5 were purified with ethyl acetate extraction, column chromatography, and high-performance liquid chromatography and identified as hexahydropyrrolo[1,2-a]pyrazine-1,4-dione and 3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione based on liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, and nuclear magnetic resonance analyses. The active algicidal compounds S-5A (hexahydropyrrolo[1,2-a]pyrazine-1,4-dione) and S-5B (3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione) displayed high levels of algicidal activity against M. aeruginosa 9110, with LD50 values of 5.7 and 19.4μg/ml, respectively. This is the first report of 3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione as an algicidal compound. Compounds S-5A and S-5B also induced obvious morphological changes in M. aeruginosa 9110. In cocultures of M. aeruginosa 9110 and Bacillus sp. Lzh-5, the cell density of Bacillus sp. Lzh-5 and the concentrations of S-5A and S-5B correlated positively with the algicidal activity. Our results indicate that strain Lzh-5 and its two algicidal compounds are potentially useful for controlling cyanobacterial blooms in Lake Taihu. Springer-Verlag Berlin Heidelberg, 2014 Algicidal bacteria nationallicence Cyanobacterial bloom nationallicence Microcystis aeruginosa nationallicence 3-Isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione nationallicence Hexahydropyrrolo[1,2-a]pyrazine-1,4-dione nationallicence Li Zhenghua State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, China aut Geng Mengxin State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, China aut Yang Hong State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 981-990 0175-7598 99:2<981 2015 99 253 https://doi.org/10.1007/s00253-014-6043-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6043-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Zhenghua State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, China aut NATIONALLICENCE 700 1- Geng Mengxin State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, China aut NATIONALLICENCE 700 1- Yang Hong State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/2(2015-01-01), 981-990 0175-7598 99:2<981 2015 99 253